|Table of Contents|

New tumor-specific HPL lymphocytes induce expression of apoptosis signals in gastric cancer cell strains

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 23
Page:
4306-4310
Research Field:
Publishing date:

Info

Title:
New tumor-specific HPL lymphocytes induce expression of apoptosis signals in gastric cancer cell strains
Author(s):
XU Hongyong1; XU Li2
1.Department of General Surgery，the 986th Hospital of Airforce,PLA，Shaanxi Xi'an 710054,China；2.Digestive Department,Xiang'an Hospital,Xiamen University,Fujian Xiamen 361000,China.
Keywords:
garstic carcinoma; scFv; T lymphocytes; apoptosis
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.23.003
Abstract:
Objective:To study the effect of new tumor-specific human peripheral lymphosytals（HPL） on apoptosis of gastric cancer cells.Methods:In order to prepare a novel tumor-specific HPL,we first isolated peripheral blood mononuclear cells(PBMC) from peripheral blood,and then introduced the recombinant vector scFv-CD3ζ-pcDNA3.0 into PBMC by liposome-mediated cell transfection technology,and successfully obtained the target cells.HGC27 and SGC7901 cells were treated with new tumor-specific HPL,and the expression of apoptosis signal was detected by Annexin V-FITC flow cytometry.In addition,the changes of Bcl-2,Bcl-x and Bax apoptosis proteins in these two groups of gastric cancer cells were detected by Western blot to study the regulatory effect of new tumor-specific HPL on gastric cancer cell apoptosis.Results:The new tumor-specific HPL was used to treat gastric cancer HGC27 and SGC7901 cells,and the expression of apoptosis signal Annexin V was detected.It was found that the early and late apoptosis rate of HGC27 cells was (62.46±4.1)%,which was higher than that of SGC7901 cells (15.40±4.8)% (P<0.05).By Western blot analysis,it was found that the expression level of apoptosis-promoting protein Bax in HGC27 cells increased significantly,while the expression level of Bax in SGC7901 cells decreased significantly.In addition,the expression levels of anti-apoptosis proteins Bcl-2 and Bcl-x decreased significantly in HGC27 cells,but increased significantly in SGC7901 cells.Conclusion:The novel tumor-specific HPL can induce apoptosis of gastric cancer HGC27 cells,increase the expression of apoptosis protein Bax,and inhibit the expression of Bcl-2 and Bcl-x,thus inducing apoptosis of gastric cancer cells.The results of this study are expected to achieve effective treatment of gastric cancer.

References:

［1］MATSSUOKA T,YASHIRO M.Novel biomarkers for early detection of gastric cancer［J］.World J Gastroenterol,2023,29(17):2515-2533.
［2］ILGIZ GAREEV,AAMIR AHMAD,WANG JQ,et al.Gastric juice non-coding RNAs as potential biomarkers for gastric cancer［J］.Front Physiol,2023，14:1179582.
［3］LAHOUD RM,O' SHEA A,EL-MOUHAYYAR C,et al.Tumour markers and their utility in imaging of abdominal and pelvic malignancies［J］.Clin Radiol,2021,6(2):99-107.
［4］BOSCH X,MOLINA R,MARRADES R,et al.Tumour markers with clinically controlled cut-offs for suspected cancer［J］.Eur J Clin Invest,2021,51(7):e13523.
［5］JELSKI W,MROCZKO B.Molecular and circulating biomarkers of gastric cancer［J］.Int J Mol Sci，2022,23(14):7588-7601.
［6］MURRAY MJ,SCARPINI CG,COLEMAN N.Serum tumour markers for testicular cancer recurrence［J］.Nat Rev Urol,2023,20(8):459-460.
［7］徐宏勇，徐立，李开宗.一种新型胃肠道癌特异性单链抗体的表达及其在胃癌中的检测［J］.胃肠病学和肝病学杂志，2011,20(8):754-757. XU HY,XU L,LI KZ.Expression of a new scFv directed towards gastrointestinal carcinoma［J］.Chin J Gastroentrol Heptol,2011,20(8):754-757.
［8］BUMBAT M,WANG M,LIANG W,et al.Effects of Me2SO and trehalose on the cell viability,proliferation,and Bcl-2 family gene(BCL-2,BAX,and BAD) expression in cryopreserved human breast cancer cells［J］.Biopreserv Biobank,2020,18(1):33-40.
［9］KALONI D,DIEPSTRATEN ST,STRASSRE A,et al.BCL-2 protein family:Attractive targets for cancer therapy［J］.Apoptosis,2023,28(1-2):20-38.
［10］ΖHANG Y,YANG X,GE X,et al.Puerarin attenuates neurological deficits via Bcl-2/Bax/cleaved caspase-3 and Sirt3/SOD2 apoptotic pathways in subarachnoid hemorrhage mice［J］.Biomed Pharmacother,2019,109(1):726-733.
［11］徐立，徐宏勇，樊代明.胃肠道肿瘤靶向抗癌胚抗原单链抗体与T细胞表面受体基因的融合分子构建［J］.中华消化杂志, 2004,24(2):75-77. XU L,XU HY,FAN DM.Constructing a fusion molecular with T cell receptor for activaton of carcinoembryonic actigen specific cytotoxic T lymphocytes to gastrointestinal tumors［J］.Chin J Dig,2004,24(2):75-77.
［12］MR 格林，J 萨姆布鲁克.分子克隆实验指南［M］.贺福初译.第4版.北京:科学出版社,2017:938-946. MR GREEN, J SAM BROOKER.The condensed protocols from molecular coning:A laboratory manual［M］.HE FC translate.4th edition.Beijing:Science Publishing House,2017:938-946.
［13］徐宏勇,徐立.一种特异性靶向性淋巴细胞对荷瘤裸鼠的治疗观察［J］.中国普外基础与临床杂志,2018,25(3):283-288. XU HY,XU L.Obsevation of therapy a new specific cytotoxic T lymphocytes towards tumor bearing nude mice［J］.Chin J Bases Clin General Surg,2018,25(3):283-288.
［14］SCHLUCK M,HAMMINK R,FIGDOR CG,et al.Biomaterial-based activation and expansion of tumor-specific T cells［J］.Front Immunol,2019,10(3):931-942.
［15］KUMARI S,DHAPOLA R,REDDY DH.Apoptosis in Alzheimer's disease:Insight into the signaling pathways and therapeutic avenues［J］.Apoptosis,2023,28(7-8):943-957.
［16］KUPCHO K,SHULTZ J,HURST R,et al.A real-time,bioluminescent annexin V assay for the assessment of apoptosis［J］.Apoptosis,2019,24(1-2):184-197.
［17］KUMAR S,FAIRMICHAEL C,LONGLEY DB,et al.The multiple roles of the IAP super-family in cancer［J］.Pharmacol Ther,2020,214:107610.
［18］LI Q,ZHANG W.Progress in anticancer drug development targeting ubiquitination-related factors［J］.Int J Mol Sci,2022,23(23):15104-15119.
［19］SHARMA VK,SINGH TG,SINGH S,et al.Apoptotic pathways and Alzheimer's disease:Probing therapeutic potential［J］.Neurochem Res,2021,46(12):3103-3122.
［20］KHODAVIRDIPOUR A,JAMSHIDI F,NEJAD HR,et al.To study the anti-cancer effects of shigella flexneri in AspC-1 pancreatic cancer cell line in approach to Bax and Bcl-2 genes［J］.J Gastrointest Cancer,2021,52(2):593-599.
［21］RATATAJCZAK K,GLATZEL-PLUCINSKA N,RATAJCZAK B,et al.Effect of resveratrol treatment on human pancreatic cancer cells through alterations of Bcl-2 family members［J］.Molecules,2021,26(21):6560.
［22］EL-SAWY ER,EL-SHAHID ZA,SOLIMAN AAF,et al.Synthetic analogs of marine alkaloid aplysinopsin suppress anti-apoptotic protein BCL2 in prostate cancer［J］.Molecules,2022,28(1):109-126.
［23］BRINKMANN K,NG AP,DE GRAAF CA,et al.What can we learn from mice lacking pro-survival BCL-2 proteins to advance BH3 mimetic drugs for cancer therapy［J］.Cell Death Differ,2022,29(6):1079-1093.
［24］COSENTINO K,HERTLEIN V,JENNER A,et al.The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation［J］.Mol Cell,2022,82(5):933-949.
［25］LI M,WANG D,HE J,et al.Bcl-XL:A multifunctional anti-apoptotic protein［J］.Pharmacol Res,2020,151(1):104547.
［26］HOFFMEISTER-WITTMANN P,MOCK A,NICHETTI F,et al.Bcl-xL as prognostic marker and potential therapeutic target in cholangiocarcinoma［J］.Liver Int,2022,42(12):2855-2870.
［27］SCIMECA M,ROVELLA V,PALUMBOL V,et al.Programmed cell death pathways in cholangiocarcinoma:Opportunities for targeted therapy［J］.Cancers(Basel),2023,15(14):3638-3668.

Memo

Memo:
National Natural Science Foundation of China（No.39900068）;国家自然科学基金(编号:39900068);陕西省西安市科技攻关计划项目(编号:HM1117-5)
Last Update: 2023-10-31