|Table of Contents|

Study of oxygen content affecting the chemosensitivity of liver cancer by regulating protein SUMOylation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 23
Page:
4295-4300
Research Field:
Publishing date:

Info

Title:
Study of oxygen content affecting the chemosensitivity of liver cancer by regulating protein SUMOylation
Author(s):
YAN Yulan1; ZHANG Chunyan2; 3; SHAO Zhijiang2; CHEN Hekai2; ZHANG Shupeng2; ZHAO Hui2; WU Wenhan2; 3; 4
1.Department of Hematology and Oncology;2.Department of General Surgery,Tianjin Fifth Central Hospital,Tianjin 300450,China;3.Tianjin Key Laboratory of Epigenetic Organ Development of Preterm Infants,Tianjin 300450,China;4.Department of General Surgery,Peking University First Hospital,Beijing 100034,China.
Keywords:
liver cancer; small ubiquitin-related modified protein; hypoxia; hyperoxia; chemosensitivity
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.23.001
Abstract:
Objective:To analyze the effect of oxygen content on the chemosensitivity of liver cancer from the perspective of protein SUMOylation modification,and provide experimental basis for chemotherapy sensitization of liver cancer patients in the future.Methods:The human-derived hepatoma cell line Hep3B was cultured in hypoxia,normoxia and hyperoxia,and Western Blot was used to detect the protein expression level of small ubiquitin-like-related modified protein 1(SUMO1),SUMO2/3,and sex-determining region Y-box2(Sox2) and octamer-binding transcription factor 4(Oct4).The formation rate of Hep3B cell clone spheres was observed.Then,cisplatin was added to Hep3B cell culture medium,3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide(MTT) method was used to detect the median lethal dose of tumor cells.The content of lactate dehydrogenase(LDH) was detected by enzyme linked immunosorbent assay(ELISA),and the apoptosis rate was detected by flow cytometry.Results:Both hypoxia and hyperoxia did not affect the increase of covalent SUMO1 products(P＞0.05).Hypoxia could significantly increase covalent SUMO2/3 products,but hyperoxia temporarily increased covalent SUMO2/3 products and then decreased again.Hypoxia can increase the formation rate of clonal spheres and the protein expressions of cell stemness maintenance proteins Sox2 and Oct4(P＜0.05),while hyperoxia decreased the formation rate of clonal spheres and the protein expressions of Sox2 and Oct4(P＜0.05).Hypoxia can increase the median lethal dose of Hep3B cells to cisplatin(P＜0.05),reduce the level of LDH and apoptosis rate(P＜0.05),and hyperoxia can reduce the median lethal dose of Hep3B cells to cisplatin(P＜0.05),increased LDH level and apoptosis rate(P＜0.05).Conclusion:Hypoxia can increase the stemness of liver cancer cells and reduce the sensitivity of chemotherapy by increasing the level of protein SUMOylation.Hyperoxia can reduce the stemness of liver cancer cells and improve the sensitivity of chemotherapy by reducing the level of protein SUMOylation.

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Memo

Memo:
National Natural Science Foundation of China（No.82272880）;国家自然科学基金项目（编号：82272880）；天津市卫生健康委员会科技项目（编号：TJWJ2022XK041，TJWJ2022XK042， TJWJ2022MS051，TJWJ2022QN101）；天津市医学重点学科（专科）建设项目资助（编号：TJYXZDXK-079D）
Last Update: 2023-10-31