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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 22

Page:

4136-4141

Research Field:

Publishing date:

Info

Title:

miR-196 inhibits the invasion of osteosarcoma cells by regulating epithelial-mesenchymal transition

Author(s):

ZHANG Jingyong; HE Ming

Department of Orthopaedic Surgery,Shengjing Hospital,China Medical University,Liaoning Shenyang 110000,China.

Keywords:

osteosarcoma; microRNAs; epithelial-mesenchymal transition; invasion

PACS:

R738.1

DOI:

10.3969/j.issn.1672-4992.2023.22.007

Abstract:

Objective:To explore the differential expression of miR-196 in osteosarcoma and adjacent tissues,and research into the role of miR-196 on SMAD4 and epithelial-mesenchymal transformation related factors in osteosarcoma MG-63 cells and the effect of miR-196 on the invasion ability of osteosarcoma MG-63 cells.Methods:RT-qPCR was used to detect the differential expression of miR-196 in osteosarcoma and adjacent tissues of 20 patients.The Targetscan and miRDB database was used to search for the binding site of miR-196 sequence and SMAD4 sequence.Spearman correlation analysis was used to analyze the correlation between miR-196 and SMAD4 expression.The regulation of miR-196 and SMAD4 was verified by dual luciferase reporting assay.The expression relationships of miR-196 and SMAD4 and epithelial-mesenchymal transformation related factors at RNA level and protein level were detected by RT-qPCR and Western blotting respectively.Transwell assay was used to detect the effect of miR-196 on the invasion ability of osteosarcoma MG-63 cells.Results:miR-196 was down-regulated in osteosarcoma tissues compared with adjacent tissues,and the expression of miR-196 and SMAD4 was negatively correlated in osteosarcoma (Y=-0.241 8*X+1.188).Overexpression of miR-196 can inhibit the expressions of SMAD4,N-cadherin and Vimentin at both RNA and protein levels,it can also promote the expression of E-cadherin,and inhibit the invasion ability of osteosarcoma MG-63 cells.Conclusion:miR-196 can inhibit the progression of epithelial-mesenchymal transformation by down-regulating SMAD4,thus inhibiting the invasion ability of osteosarcoma MG-63 cells.

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