|Table of Contents|

The role of myotubularin 1 in the migration and invasion of liver cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 22
Page:
4109-4114
Research Field:
Publishing date:

Info

Title:
The role of myotubularin 1 in the migration and invasion of liver cancer cells
Author(s):
KOU Xueying1; ZHANG Ge2; ZHOU Xiaorong1; XIONG Lan1; LIU Guochen1; LIU Dan1; LYU Zhuomin3
1.Department of Pain Treatment；2.Department of Hematology；3.Department of Burn and Plastic Surgery,the Second Affiliated Hospital,Airforce Military Medical University,Shaanxi Xi'an 710038,China.
Keywords:
MTM1; migration; invasion; EMT; liver cancer
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.22.003
Abstract:
Objective:To explore the expression and biological functions of MTM1 (myotubularin 1) in the promotion of liver cancer metastasis.Methods:Bioinformatic analysis and immunohistochemistry staining assay were applied to determine the expression and prognostic significance of MTM1 in liver cancer.The effect of forced MTM1 expression on migration of liver cancer cells was determined by wound healing and Transwell migration assays.The effect of forced MTM1 expression on invasion of liver cancer cells was determined by Transwell invasion assay.The effect of forced MTM1 expression on the expressions of epithelial-mesenchymal transition(EMT) markers was determined by real-time-PCR and Western blot analysis.The effect of forced MTM1 expression on the in vivo metastasis of liver cancer cells was determined by nude mice lung metastasis model.Results:Bioinformatic analysis and immunohistochemistry staining assay revealed that MTM1 expression was markedly decreased in tumor tissues of liver compared non-tumor tissues,especially in metastatic tumor tissues.In addition,patients with low MTM1 expression had significant poorer survival than patients with high MTM1 expression.The migration ability of liver cancer cells was significantly suppressed when MTM1 was overexpressed.The invasion ability of liver cancer cells was significantly decreased when MTM1 was overexpressed.The progress of EMT was inhibited by MTM1 overexpression,as evidenced by increased expressions of epithelial marker (E-cadherin and ZO-1) and decreased expressions of mesenchymal marker (N-cadherin and Vimentin).Forced MTM1 expression significantly impaired the in vivo metastasis of liver cancer cells.Conclusion:MTM1 downregulation promotes the progression of EMT and thus migration and invasion in liver cancer cells.

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Memo

Memo:
National Natural Science Foundation of China(No.82172922）；国家自然科学基金面上项目(编号：82172922)
Last Update: 1900-01-01