|Table of Contents|

The expression of GPX4 in renal cell carcinoma tissues and its impact on the proliferation,migration and invasion ability of ACHN cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 22
Page:
4105-4109
Research Field:
Publishing date:

Info

Title:
The expression of GPX4 in renal cell carcinoma tissues and its impact on the proliferation,migration and invasion ability of ACHN cells
Author(s):
HAN Jingya1; DUAN Yatao2; ZHANG Xiaoyu2; REN Zongtao2
1.Department of Nuclear Medicine;2.Department of Urology,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China.
Keywords:
RCC; GPX4; ACHN cells; proliferation; migration; invasion
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2023.22.002
Abstract:
Objective:To explore the expression of glutathione peroxidase 4 (GPX4) in renal cell carcinoma (RCC) tissues and ACHN cells,also to investigate its effect on the proliferation,migration and invasion of ACHN cells.Methods:The expression of GPX4 in RCC tissues and normal renal tissues was assessed by immunohistochemistry and reverse transcription-quantitative real-time PCR (qRT-PCR).shRNA plasmid with GPX4 knockdown was constructed and transfected into ACHN cells for functional experiments to detect the effect of GPX4 knockdown on the malignant biological behavior of ACHN cells.The effect of GPX4 knockdown on the expression of GPX4 protein in ACHN cells was detected by Western blot.Results:The expression of GPX4 protein and mRNA in RCC tissues was significantly higher than that in normal renal tissues (P<0.01).Transfection of shGPX4 into ACHN cells could knock down GPX4 gene and protein expression (P<0.05).Functional experiments demonstrated that proliferation (P<0.05),migration (P<0.05) and invasion (P<0.05) ability was significantly inhibited by knockdown of GPX4 in ACHN cells.Conclusion:GPX4 was highly expressed in RCC tissues and ACHN cells and knockdown of GPX4 could inhibit the malignant biological behavior of ACHN cells,we preliminarily inferred that GPX4 may serve as an oncogene in RCC progression.

References:

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