|Table of Contents|

let-7b-3p targeting and regulating ANKRD49 inhibits the invasion and migration of nasopharyngeal carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 22
Page:
4097-4104
Research Field:
Publishing date:

Info

Title:
let-7b-3p targeting and regulating ANKRD49 inhibits the invasion and migration of nasopharyngeal carcinoma cells
Author(s):
WANG Yi; GOU Xiaoxia; ZHU Lin; HUANG Gang; CHEN Nian; ZHOU Kai; SHEN Shasha
Department of Head and Neck Oncology,the Second Affiliated Hospital of Zunyi Medical University,Guizhou Zunyi 563003,China.
Keywords:
let-7b-3p; ANKRD49; nasopharyngeal carcinoma; invasion; migration
PACS:
R739.63
DOI:
10.3969/j.issn.1672-4992.2023.22.001
Abstract:
Objective:To explore the potential mechanism by which let-7b-3p regulates the expression of ankyrin repeat domain 49 (ANKRD49) and affects the invasion and migration of nasopharyngeal carcinoma (NPC) cells.Methods:GEO and TCGA databases were used to analyze the expression level of let-7b in NPC and head and neck tumor tissues and its relationship with clinicopathological parameters.The expression level of ANKRD49 in NPC and nasopharyngeal inflammatory tissues was detected by immunohistochemical technique.RT-PCR detected let-7b-3p expression in NPC cell lines.Transwell invasion assay and scratch assay were used to detect the role of let-7b-3p in the invasion and migration of NPC cells.The targeting relationship between let-7b-3p and ANKRD49 was verified by dual luciferase gene reporter assay.RT-PCR,Western Blot and reply experiments verified the regulatory effect of let-7b-3p on ANKRD49.Results:Based on NPC miRNA microarray analysis,let-7b-3p expression was down-regulated in NPC tissues compared with normal nasopharyngeal tissues,and the expression level of let-7b-3p was correlated with tumor invasion depth and lymph node stage (P＜0.05).Immunohistochemistry showed that ANKRD49 was highly expressed in NPC tissues.The results of invasion and migration experiments showed that the let-7b-3p mimic group and the inhibitor group respectively inhibited and promoted the invasion ability and migration ability of NPC cells (both P＜0.05).The double-luciferase reporter assay showed that let-7b-3p mimic could inhibit the luciferase activity of ANKRD49-wt (0.35±0.08 vs 0.61±0.05,P＜0.01),but did not affect the luciferase activity of ANKRD49-mut (0.64±0.13 vs 0.57±0.13,P＞0.05).The results of RT-PCR and Western Blot showed that the expression levels of ANKRD49 mRNA and protein in the let-7b-3p inhibitor group were higher than those in the normal group and let-7b-3p mimic group.Reversion experiments found that interference with ANKRD49 affects the promoting and inhibiting effects of let-7b-3p inhibitor group and let-7b-3p mimic group on the invasion and migration of NPC cells.Conclusion:let-7b-3p regulates the expression of ANKRD49 to inhibit the invasion and migration of NPC cells.The let-7b-3p/ANKRD49 axis may be a potential therapeutic target for NPC.

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Memo:
贵州省卫生健康委科学技术基金项目(编号：gzwkj2021-053)；贵州省科技厅基础研究计划［编号：黔科合基础-ZK(2023)一般525］;贵州省遵义市科技厅项目［编号：遵市科合HZ字（2022）407号］
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