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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 21

Page:

3945-3950

Research Field:

Publishing date:

Info

Title:

Expression of TTK in NPC tissues and its influence on the biological behavior of NPC cells

Author(s):

Yunsheng¹; LI Ping¹; FENG Yiyuan²; WANG Ansheng¹; ZHANG Wensi¹; ZHAO Haiqi¹; ZHANG Xiong²; YANG Hongbin²

1.North Sichuan Medical College£¬Sichuan Nanchong 637000,China;2.Department of Otorhinolaryngology Head and Neck Surgery,Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China.

Keywords:

NPC; TTK; bioinformatics; immunohistochemistry; proliferation; apoptosis; cell cycle; apoptosis pathways

PACS:

R739.6

DOI:

10.3969/j.issn.1672-4992.2023.21.008

Abstract:

Objective:The threonine and tyrosine kinase(TTK) was selected to determine its expression in nasopharyngeal carcinoma(NPC) tissues and the effects of TTK knockdown and the biological mechanism of human NPC CNE2 cells.Methods:Using a bioinformatics approach,based on the NPC patient dataset in the GEO database,TTK,the core gene associated with the development of nasopharyngeal carcinoma was screened.Immunohistochemical experiments of TTK expression in NPC tissues and chronic NPC tissues. After the knockdown of TTK by lentiviral transfection of human NPC CNE2 cells,the effect of TTK knockdown on the proliferation of CNE2 cells was tested by CCK-8 experiment.The effect of low TTK expression on apoptosis and cycle of CNE2 cells was tested in flow cell experiments.Western Blot was used to detect the expression of BCL-2 and BAX,the key protein in the apoptosis signaling pathway,in CNE2 cells with low expression of TTK.To explore the possible mechanism of TTK in the development of NPC.Results:The core gene TTK was selected by bioinformatics methods.TTK showed high expression in NPC tissues and low expression in chronic NPC tissues.After TTK knockdown,CNE2 cell proliferation was inhibited and cell apoptosis was increased.Reduced G1 phase of the cell cycle and increased S and G2 phases,suggesting that TTK knockdown caused cell arrest in the S/G2 phase.Silencing of TTK led to a decrease in BCL-2 protein expression and an elevated BAX protein expression in CNE2 cells.Conclusion:TTK is highly expressed in NPC tissues.TTK knockdown affected the expression of BCL-2 and BAX of proliferation,apoptosis,cycle and apoptotic pathway in NPC CNE2 cells,indicating that TTK may participate in the development and development of NPC through the apoptotic pathway.

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Last Update: 2023-09-28