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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 21

Page:

3939-3945

Research Field:

Publishing date:

Info

Title:

Research on the mechanism of Circ_0058063 in regulating the proliferation,migration and invasion of bladder cancer cells by targeting SOX4 through miR-338-3p

Author(s):

WANG Qian; SHEN Wei; SUN Bin; LI Yinnan; SHEN Jie; XIA Zhizhong; ZHANG Yunwei

Department of Urology，Chongming Hospital Affiliated to Shanghai University of Medicine & Health Sciences,Shanghai 202150,China.

Keywords:

Circ_0058063; miR-338-3p; bladder cancer; proliferation; apoptosis; migration; invasion

PACS:

R737.14

DOI:

10.3969/j.issn.1672-4992.2023.21.007

Abstract:

Objective:To investigate the effects of Circ_0058063 on the proliferation,migration and invasion of bladder cancer cells by targeting SOX4 through miR-338-3p.Methods:qRT-PCR and Western Blot were applied to detect the expression of normal human urothelium cell line SV-HUC-1 cells,T24 bladder cancer cells Circ_0058063,miR-338-3p and SOX4.si-NC,si-Circ_0058063,si-Circ_0058063+inhibitor NC,si-Circ_0058063+miR-338-3p inhibitor were transfected into T24 cells,and they were named as si-NC group,si-Circ_0058063 group,si-Circ_0058063+inhibitor NC group,and si-Circ_0058063+miR-338-3p inhibitor group respectively.T24 cells without any treatment were recorded as NC group.The dual luciferase reporter gene experiment was applied to detect the relationship between Circ_0058063,miR-338-3p and SOX4.qRT-PCR was applied to detect the expression of Circ_0058063 and miR-338-3p in T24 cells;CCK-8 method was applied to detect the proliferation of T24 cells.Flow cytometry was applied to detect the apoptosis rate of T24 cells.Transwell was applied to detect the number of invasion and migration of T24 cells.Western Blot was applied to detect the protein levels of SOX4,E-cadherin,Vimentin,and N-cadherin in T24 cells.Results:Compared with SV-HUC-1 cells,the levels of Circ_0058063 and SOX4 in T24 cells were obviously up-regulated(P<0.05),while the expression of miR-338-3p was obviously down-regulated(P<0.05).Compared with the NC group and si-NC group,the OD450 value, migration,invasion cell count,Circ_0058063 expression level,SOX4,N-cadherin,and Vimentin protein levels of T24 cells in the si-Circ_0058063 group were obviously reduced(P<0.05),the apoptosis rate of T24 cells,miR-338-3p expression,and E-cadherin protein level were obviously increased(P<0.05).The down-regulation of miR-338-3p expression reversed the inhibitory effect of silencing Circ_0058063 on T24 cell proliferation,migration and invasion.Circ_0058063 targeted the miR-338-3p/SOX4 axis.Conclusion:To silent Circ_0058063 may inhibit the expression of SOX4 by up-regulating miR-338-3p,thereby affecting the proliferation,migration and invasion of bladder cancer cells.

References:

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Last Update: 2023-09-28