|Table of Contents|

The effects and mechanisms of cancer-associated fibroblast-derived SULF1 on the biological behavior of non-small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 21
Page:
3933-3938
Research Field:
Publishing date:

Info

Title:
The effects and mechanisms of cancer-associated fibroblast-derived SULF1 on the biological behavior of non-small cell lung cancer
Author(s):
ANG Zhengbo; SHEN Yuguang; SHU Dejun
Department of Thoracic Surgery,Zunyi First People’s Hospital（the Third Affiliated Hospital of Zunyi Medical University）,Guizhou Zunyi 563000,China.
Keywords:
ancer-associated fibroblasts; sulfatase 1; Wnt/β-catenin pathway; non-small cell lung cancer
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.21.006
Abstract:
Objective:To explore the effects of sulfatase1(SULF1) derived from cancer-associated fibroblasts(CAFs) on the progression of non-small cell lung cancer(NSCLC).Methods:Normal fibroblasts(NFs) and CAFs were isolated from paracancer tissue and NSCLC tissue,NFs-CM and CAFs-CM were co-cultured with NSCLC cells.SULF1 expression in CAFs was intervented.Cell proliferation was detected by CCK8,invasion was detected by Transwell,and epithelial-mesenchymal transition(EMT)-related proteins(E-cadherin,N-cadherin) and core proteins of Wnt/β-catenin pathway(Wnt3,β-catenin) were detected by Western Blot.The tumor formation model of nude mice was established to evaluate the effects of SULF1 secreted by CAFs on tumor growth.Results:Compared with NFs-CM,CAFs-CM promoted proliferation,invasion and EMT of NSCLC cells(P<0.01).Compared with CAFssi-NC-CM group,cell proliferation,invasion and EMT were inhibited in CAFssi-SULF1-CM group(P<0.01).Compared with CAFsvector-CM group,overexpression of SULF1 could up-regulate the expression of Wnt3 and β-catenin(P<0.001).However,the effects of SULF1 overexpression in CAFs on NSCLC cells was partially saved by Wnt/β-catenin pathway inhibitors.In vivo studies showed that CAFs promote tumor growth and Ki67 expression,while SULF1 knockdown inhibits tumor growth and Ki67 expression in tumor tissues.Conclusion:CAFs-derived SULF1 induces NSCLC progression by activating the Wnt/β-catenin pathway.

References:

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贵州省遵义市科技局科技计划项目［编号：遵市科合HZ字（2021）272号］
Last Update: 2023-09-28