|Table of Contents|

Influence of LINC01296 on cisplatin resistance in ovarian cancer by regulating Wnt/β-catenin pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 20
Page:
3770-3776
Research Field:
Publishing date:

Info

Title:
Influence of LINC01296 on cisplatin resistance in ovarian cancer by regulating Wnt/β-catenin pathway
Author(s):
LIN Fangting; WU Qingyu; WANG Ru; LI Bo
Department of Gynecology,Hainan Women and Children Medical Center,Hainan Haikou 570100,China.
Keywords:
long non-coding RNA LINC01296; Wnt/β-catenin pathway; ovarian cancer; cisplatin resistance
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2023.20.009
Abstract:
Objective:To investigate the influence of long non-coding RNA LINC01296 on cisplatin resistance in ovarian cancer by regulating Wnt/β-catenin pathway.Methods:Human ovarian cancer SKOV3 cells and SKOV3 cisplatin-resistant SKOV3/DDP cells were cultured,and the expression of LINC01296 was detected by qRT-PCR.SKOV3/DDP cells were separated into 5 groups:Control group (normal culture),si-NC group (transfected with si-NC),si-LINC01296 group (transfected with si-LINC01296),si-NC+LiCl group (transfected with si-NC+Wnt/β-catenin pathway agonist LiCl 10 mmol/L treatment),and si-LINC01296+LiCl group (transfected with si-LINC01296+10 mmol/L LiCl treatment).qRT-PCR was used to verify the transfection effect.The median inhibitory concentration (IC50) of cisplatin in SKOV3/DDP cells in each group (0,1,2,4,8,16,32 μg/mL cisplatin treatment) and proliferation activity were determined by CCK-8 method.The apoptosis of SKOV3/DDP cells in each group was detected by flow cytometry.The migration ability of SKOV3/DDP cells in each group was detected by scratch healing test.The expression of Wnt/β-catenin pathway and proliferation,apoptosis,migration and invasion-related proteins Ki67.B-cell lymphoma/leukemia gene-2-associated X protein gene (Bax),matrix metalloproteinase 9 (MMP9),N-cadherin in SKOV3/DDP cells in each group was detected by Western Blot.Results:The expression level of LINC01296 in SKOV3/DDP cells was obviously higher than that in SKOV3 cells (P＜0.05).After SKOV3/DDP cells were transfected with si-LINC01296,the expression level of LINC01296 was obviously lower than that in the control group and si-NC group (P＜0.05),indicating that the transfection was successful.Compared with the control group,the cisplatin IC50,proliferation activity,healing rate of cell scratch and invasion number of SKOV3/DDP cells all decreased in the si-LINC01296 group,the apoptosis rate increased,the protein expression of β-catenin,Ki67,MMP9,and N-cadherin decreased,and the protein expression of Bax increased (P＜0.05).Compared with si-LINC01296 group,the cisplatin IC50,proliferation activity,healing rate of cell scratch and invasion number of SKOV3/DDP cells all increased in the si-LINC01296+LiCl group,the apoptosis rate decreased,the protein expression of β-catenin,Ki67,MMP9,and N-cadherin increased,and the protein expression of Bax decreased (P＜0.05).Conclusion:Knockdown of LINC01296 can reduce cisplatin resistance in SKOV3/DDP cells,possibly by inhibiting the Wnt/β-catenin pathway.

References:

［1］SONG M,CUI M,LIU K.Therapeutic strategies to overcome cisplatin resistance in ovarian cancer［J］.Eur J Med Chem,2022,232:114205.
［2］YANG M,LU H,LIU J,et al.lncRNAfunc:a knowledgebase of lncRNA function in human cancer［J］.Nucleic Acids Res,2022,50(D1):D1295-D1306.
［3］KHAWAR MB,HAMID SE,JAN T,et al.Diagnostic,prognostic and therapeutic potential of long noncoding RNAs in cancer［J］.Mol Biol Rep,2022,49(3):2311-2319.
［4］QIN QH,YIN ZQ,LI Y,et al.Long intergenic noncoding RNA 01296 aggravates gastric cancer cells progress through miR-122/MMP-9［J］.Biomed Pharmacother,2018,97:450-457.
［5］WANG B,LIANG T,LI J.Long noncoding RNA LINC01296 is associated with poor prognosis in ESCC and promotes ESCC cell proliferation,migration and invasion［J］.Eur Rev Med Pharmacol Sci,2018,22(14):4524-4531.
［6］WANG X,WANG L,GONG Y,et al.Long noncoding RNA LINC01296 promotes cancer-cell proliferation and metastasis in urothelial carcinoma of the bladder［J］.Onco Targets Ther,2018,12:75-85.
［7］LI Y,ZHANG H,GUO J,et al.Downregulation of LINC01296 suppresses non-small-cell lung cancer via targeting miR-143-3p/ATG2B［J］.Acta Biochim Biophys Sin (Shanghai),2021,53(12):1681-1690.
［8］LIANG KY,CHUN-YU HO D,YANG HP,et al.LINC01296 promotes cancer stemness traits in oral carcinomas by sponging miR-143［J］.J Dent Sci,2023,18(2):814-821.
［9］XIAO H,LI Y,ZHANG Y,et al.Long noncoding RNA LINC01296 regulates the cell proliferation,migration and invasion in neuroblastoma［J］.Metab Brain Dis,2022,37(4):1247-1258.
［10］XU H,MAO HL,ZHAO XR,et al.MiR-29c-3p,a target miRNA of LINC01296,accelerates tumor malignancy:therapeutic potential of a LINC01296/miR-29c-3p axis in ovarian cancer［J］.J Ovarian Res,2020,13(1):31-39.
［11］XU H,ZHENG JF,HOU CZ,et al.Up-regulation of long intergenic noncoding RNA 01296 in ovarian cancer impacts invasion,apoptosis and cell cycle distribution via regulating EMT［J］.Cell Signal,2019,62:109341.
［12］ZHANG Y,WANG X.Targeting the Wnt/β-catenin signaling pathway in cancer［J］.J Hematol Oncol,2020,13(1):165-181.
［13］SONG Z,LIAO C,YAO L,et al.miR-219-5p attenuates cisplatin resistance of ovarian cancer by inactivating Wnt/β-catenin signaling and autophagy via targeting HMGA2［J］.Cancer Gene Ther,2023,30(4):596-607.
［14］GUAN J,HE J,LIAO S,et al.LncRNA UCA1 accelerates osteosarcoma progression via miR-145 and Wnt/β-catenin pathway［J］.Am J Transl Res,2022,14(9):6029-6042.
［15］LU Y,TIAN M,LIU J,et al.LINC00511 facilitates Temozolomide resistance of glioblastoma cells via sponging miR-126-5p and activating Wnt/β-catenin signaling［J］.J Biochem Mol Toxicol,2021,35(9):e22848.
［16］LIU M,ZHANG M,YIN H.Linc-ROR promotes invasion and metastasis of gastric cancer by activating epithelial-mesenchymal transition［J］.Indian J Pathol Microbiol,2022,65(3):545-550.
［17］王亚萍,余军辉,王凯.下调ARPC2通过抑制Wnt/β-catenin通路激活水平降低卵巢癌OVCA429细胞恶性转移潜能［J］.中国现代医生,2021,59(19):38-43. WANG YP,YU JH,WANG K.Down-regulated ARPC2 reduces the malignant metastasis potential of OVCA429 cells in ovarian cancer by inhibiting the activation level of Wnt/β-catenin pathway［J］.Chinese Modern Doctors,2021,59(19):38-43.
［18］张靖,白惠娟.circBIRC6靶向miR-367-3p对卵巢癌细胞顺铂耐药性的影响［J］.中华肿瘤杂志,2021,43(10):1062-1068. ZHANG J,BAI HJ.Effect of circBIRC6 targeting miR-367-3p on cisplatin resistance of ovarian cancer cells［J］.Chinese Journal of Oncology,2021,43(10):1062-1068.
［19］STEWART C,RALYEA C,LOCKWOOD S.Ovarian cancer:an integrated review［J］.Semin Oncol Nurs，2019，35(2):151-156.
［20］ORTIZ M,WABEL E,MITCHELL K,et al.Mechanisms of chemotherapy resistance in ovarian cancer［J］.Cancer Drug Resist,2022,5(2):304-316.
［21］ZHANG Y,WANG A,ZHANG X,et al.lncRNA LINC01296 promotes oral squamous cell carcinoma development by binding with SRSF1［J］.Biomed Res Int,2021,2021:6661520.
［22］BRIDGES MC,DAULAGALA AC,KOURTIDIS A.LNCcation:lncRNA localization and function［J］.J Cell Biol,2021,220(2):e202009045.
［23］HE J,ZHU S,LIANG X,et al.LncRNA as a multifunctional regulator in cancer multi-drug resistance［J］.Mol Biol Rep,2021,48(8):1-15.
［24］KRISHNAMURTHY N,KURZROCK R.Targeting the Wnt/beta-catenin pathway in cancer:Update on effectors and inhibitors［J］.Cancer Treat Rev,2018,62:50-60.

Memo

Memo:
海南省卫生计生行业科研项目（编号：20A200035）
Last Update: 1900-01-01