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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 20

Page:

3758-3763

Research Field:

Publishing date:

Info

Title:

Influence and mechanism of Emodin on biological behavior of endometrial carcinoma cells through TGF-β1/SMAD4 pathway

Author(s):

WANG Xiaonuan; WEN Yunhua; WEI Qiuyuan; LI Tingna

Department of Obstetrics and Gynaecology,Haikou Hospital of the Maternal and Child Health,Hainan Haikou 570203,China.

Keywords:

Emodin; TGF-β1/SMAD4 pathway; endometrial carcinoma cells; proliferation; apoptosis; migration and invasion

PACS:

R737.33

DOI:

10.3969/j.issn.1672-4992.2023.20.007

Abstract:

Objective:To investigate the influence and mechanism of Emodin on proliferation,migration,invasion and epithelial mesenchymal transformation (EMT) of endometrial carcinoma cells.Methods:Different concentrations of Emodin (0.5~10.0 μmol/L) were used to treat human endometrial carcinoma HEC-1B cells respectively,and cell proliferation was detected to determine the optimal drug concentration.HEC-1B cells were divided into Control group,transforming growth factor-β1 (TGF-β1) group,Emodin group,Emodin+TGF-β1 group,and Disitertide (TGF-β1 inhibitor) group.The proliferation,apoptosis,migration and invasion of HEC-1B cells,and the expression of E-cadherin,N-cadherin,Vimentin,TGF-β1,mothers against decapentaplegic homolog 4 (SMAD4) proteins were detected respectively.A tumor bearing mouse model was established to test the effect of Emodin on the growth of endometrial carcinoma xenografts.Results:Emodin of 0.5~10.0 μmol/L could obviously inhibit the proliferation of HEC-1B cells.Emodin of 2.5 μmol/L concentration was selected for subsequent experiments.Compared with the Control group,the number of HEC-1B cell colonies,migration and invasion,and the expression levels of N-cadherin,Vimentin,TGF-β1 in TGF-β1 group increased obviously,the expression levels of E-cadherin and SMAD4 decreased (P＜0.05),the number of HEC-1B cell colonies,migration and invasion,and the expression levels of N-cadherin,Vimentin and TGF-β1 reduced obviously in Emodin group and Disitertide group,the apoptosis rate and the expression levels of E-cadherin and SMAD4 increased obviously (P＜0.05).Compared with Emodin group,the number of HEC-1B cell colonies,migration and invasion,and the expression levels of N-cadherin,Vimentin and TGF-β1 increased obviously in Emodin+TGF-β1 group,the apoptosis rate and the expression levels of E-cadherin and SMAD4 reduced obviously (P＜0.05).Emodin was able to obviously inhibit the growth of transplanted tumor.Conclusion:Emodin inhibits the growth of endometrial carcinoma by regulating TGF-β1/SMAD4 pathway.

References:

[1] HUVILA J,PORS J,THOMPSON EF,et al.Endometrial carcinoma:molecular subtypes,precursors and the role of pathology in early diagnosis［J］.J Pathol,2021,253(4):355-365.
[2] 杨宝芹,刘高仁,邵艳社,等.白术多糖通过调控线粒体凋亡途径诱导人子宫内膜癌RL95-2细胞凋亡［J］.中国免疫学杂志,2022,38(12):1458-1462. YANG BQ,LIU GR,SHAO YS,et al.Apoptosis of human endometrial carcinoma RL95-2 cells induced by polysaccharides from atractylodes atractylodes by regulating mitochondrial apoptosis pathway［J］.Chinese Journal of Immunology,2022,38(12):1458-1462.
[3] YANG T,HUANG T,ZHANG D,et al.TGF-β receptor inhibitor LY2109761 enhances the radiosensitivity of gastric cancer by inactivating the TGF-β/SMAD4 signaling pathway［J］.Aging (Albany NY),2019,11(20):8892-8910.
[4] LONG H,CHEN H,YAN J,et al.Emodin exerts antitumor effects in ovarian cancer cell lines by preventing the development of cancer stem cells via epithelial mesenchymal transition［J］.Oncol Lett,2022,23(3):95-105.
[5] GALIARDI-CAMPOY AEB,MACHADO FC,CARVALHO T,et al.Effects of photodynamic therapy mediated by emodin in cervical carcinoma cells［J］.Photodiagnosis Photodyn Ther,2021,35(1):102394.
[6] JIANG J,ZHOU N,YING P,et al.Emodin promotes apoptosis of human endometrial cancer through regulating the MAPK and PI3K/ AKT pathways［J］.Open Life Sci,2019,13(1):489-496.
[7] CHEN HY,CHIANG YF,HUANG CY,et al.Spirulina phycocyanin extract and its active components suppress epithelial-mesenchymal transition process in endometrial cancer via targeting TGF-beta1/SMAD4 signaling pathway［J］.Biomed Pharmacother,2022,152(1):1-8.
[8] 刘松,齐放,赵宇,等.青蒿琥酯对人结肠癌细胞侵袭转移及TGF-β1/Smad4信号通路表达的影响［J］.中国医科大学学报,2019,48(1):34-38. LIU S,QI F,ZHAO Y,et al.Effects of artesunate on invasion and metastasis of human colon cancer cells and expression of TGF-β1/Smad4 signaling pathway［J］.Journal of China Medical University,2019,48(1):34-38.
[9] 刘亚楠,王炳先,路臣桂,等.紫草素对子宫内膜癌细胞增殖、凋亡和顺铂耐药性的影响［J］.中成药,2021,43(8):2219-2224. LIU YN,WANG BX,LU CG,et al.Effects of Shikotin on proliferation,apoptosis and cisplatin resistance of endometrial cancer cells［J］.Chinese Patent Medicine,2021,43(8):2219-2224.
[10] 杨亚男，郭剑锋，蔡晶，等.二甲双胍在早期子宫内膜癌中的应用［J］.现代肿瘤医学，2021,29（12）：2173-2176. YANG Yanan,GUO Jianfeng,CAI Jing,et al.Metformin for conservative treatment of early endometrial cancer［J］.Modern Oncology,2021,29（12）：2173-2176.
[11] 齐曼，芦秋彤，刘少卿，等.白蛋白结合型紫杉醇联合阿帕替尼辅助中药治疗晚期子宫内膜癌疗效观察［J］.现代肿瘤医学，2021,29（23）：4176-4180. QI Man,LU Qiutong,LIU Shaoqing,et al.Clinical effect of albumin binding paclitaxel combined with apatinib in the auxiliary Chinese medicine treatment of advanced endometrial cancer［J］.Modern Oncology,2021,29（23）：4176-4180.
[12] HU Q,YAO J,WU X,et al.Emodin attenuates severe acute pancreatitis-associated acute lung injury by suppressing pancreatic exosome-mediated alveolar macrophage activation［J］.Acta Pharm Sin B,2022,12(10):3986-4003.
[13] ZOU G,ZHANG X,WANG L,et al.Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription［J］.Theranostics,2020,10(15):6839-6853.
[14] 刘欣,魏炜,樊钦,等.大黄素通过抑制半乳糖凝集素-3诱导甲状腺癌细胞内质网应激［J］.中华实验外科杂志,2022,39(7):1273-1277. LIU X,WEI W,FAN Q,et al.Emodin induces endoplasmic reticulum stress in thyroid cancer cells by inhibiting galactolectin 3［J］.Chinese Journal of Experimental Surgery,2022,39(7):1273-1277.
[15] 王赫，董丽，邵明敬，等.芦荟大黄素纳米脂质体介导光动力疗法抑制人口腔癌CAL-27细胞增殖并促进其自噬［J］.现代肿瘤医学，2021,29（05）：719-723. WANG He,DONG Li,SHAO Mingjing,et al.Aloe-emodin nanoliposome-mediated photodynamic therapy inhibits proliferation and promotes autophagy of human oral cancer CAL-27 cells［J］.Modern Oncology,2021,29（05）：719-723.
[16] 薛飞,董传莹,田莉,等.芦荟大黄素调控miR-30b促进子宫内膜癌细胞自噬的研究［J］.中国比较医学杂志,2021,31(10):61-67. XUE F,DONG CY,TIAN L,et al.Effects of aloe emodin on the autophagy of endometrial cancer cells［J］.Chinese Journal of Comparative Medicine,2021,31(10):61-67.
[17] DING N,ZHANG H,SU S,et al.Emodin enhances the chemosensitivity of endometrial cancer by inhibiting ROS-mediated cisplatin-resistance［J］.Anticancer Agents Med Chem,2018,18(7):1054-1063.
[18] 李瑞锋,崔燕,曾湘晖,等.桔梗皂苷D基于TGF-β/Smad通路调控上皮-间质转化对人子宫内膜癌Ishiwaka细胞迁移及侵袭的作用［J］.西部医学,2022,34(5):633-638. LI RF,CUI Y,ZENG XH,et al.Effects of platycodon platycodon D on epithelial-mesenchymal transformation on migration and invasion of human endometrial carcinoma Ishiwaka cells based on TGF-β/Smad pathway［J］.Western Medicine,2022,34(5):633-638.
[19] 田滢舟,陈思达,梁雪芳,等.莪术醇通过上调miR-214抑制TGF-β诱导的RL-95细胞生长及纤维化相关蛋白表达［J］.中国病理生理杂志,2021,37(2):328-333. TIAN YZ,CHEN SD,LIANG XF,et al.Curcumin inhibits TGF-β-induced RL-95 cell growth and fibrosis-related protein expression by up-regulating miR-214［J］.Chinese Journal of Pathophysiology,2021,37(2):328-333.
[20] CHEN MJ,WANG YC,WANG L,et al.PD-L1 expressed from tumor cells promotes tumor growth and invasion in lung cancer via modulating TGF-β1/SMAD4 expression［J］.Thorac Cancer,2022,13(9):1322-1332.
[21] 杨子霖,王志莲.调控TGF-β1/Smad4通路对宫颈癌SiHa细胞自噬基因LC3的影响［J］.现代肿瘤医学,2021,29(14):2400-2404. YANG ZL,WANG ZL.Regulation of TGF-β1/Smad4 pathway on autophagy gene LC3 in cervical cancer SiHa cells［J］.Modern Oncology,2021,29(14):2400-2404.
[22] 李雨珊,张仁静,侯维,等.过/降表达miR-4746的食管鳞癌细胞增殖、迁移、侵袭能力及TGF-β/Smad通路蛋白表达观察［J］.山东医药,2022,62(5):30-34. LI YS,ZHANG RJ,HOU W,et al.Proliferation,migration,invasion and expression of TGF-β/Smad pathway protein in esophageal squamous cell cells with over/down expression of miR-4746［J］.Shandong Pharmaceutical,2022,62(5):30-34.

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