|Table of Contents|

Construction of endometrial carcinoma ceRNA and the effect of LINC-FAM138B on the malignant biological behavior of HEC1B cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 20
Page:
3730-3736
Research Field:
Publishing date:

Info

Title:
Construction of endometrial carcinoma ceRNA and the effect of LINC-FAM138B on the malignant biological behavior of HEC1B cells
Author(s):
LI Jinghua1; TANG Xifeng2
1.Department of Obstetrics and Gynecology;2.Oncology Department,Sanya People's Hospital,Hainan Sanya 572000,China.
Keywords:
endometrial carcinoma; competing endogenous RNA; prognosis; protein interactions; LINC-FAM138B
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.20.003
Abstract:
Objective:To construct competing endogenous RNA (ceRNA) from endometrial carcinoma (EC) and study the effect of LINC-FAM138B on the malignant biological behavior of HEC1B cells.Methods:Download the expression profile of EC patients from the TCGA database and screen for differential genes.Analyze differential genes and construct a ceRNA network.STRING combined with the Cytoscape database to perform protein interaction analysis and screen the Top10 gene,and screen key genes significantly related to prognosis.RT-qPCR experiments verified the expression levels of LINC-FAM138B,hsa-mir-429,and PRKG1 in cancer and adjacent tissues of EC patients (n=7).The cells were divided into four groups:OV LINC-FAM138B,Sh LINC-FAM138B,OV NC,Sh NC.The proliferation ability of the cells was detected by CCK-8 assay.Scratch test was used to detect the migration ability of cells,and Transwell assay was used to detect the invasion ability of cells.The ability of apoptosis was detected by flow cytometry.Results:457,65 and 1 119 differentially expressed lncRNAs,miRNAs,and mRNAs were screened.A ceRNA network with 823 regulatory relationship pairs was obtained.Combining the Top10 gene and survival analysis results,it was found that PRKG1 had the highest score among LINC-FAM138B binding hsa-mir-429 targeted genes.In survival analysis,the prognosis of EC patients with high expression of PRKG1,RBFOX3,RGS2,and SLC2A4 was significantly worse than those with low expression (P＜0.05).The results of RT-qPCR experiments showed that LINC-FAM138B and PRKG1 were lower expressed in EC cancer tissues than in adjacent tissues (P＜0.05),while hsa-mir-429 was higher expressed in EC cancer tissues (P＜0.05).Compared with OV NC group,the proliferation,migration,and invasion ability of HEC1B cells in OV LINC-FAM138B group decreased significantly (P＜0.05),while the apoptosis ability increased significantly (P＜0.01).Compared with the Sh NC group,the proliferation,migration,and invasion ability of HEC1B cells in the Sh LINC-FAM138B group significantly increased (P＜0.01),while the apoptosis ability significantly decreased (P＜0.05).Conclusion:We constructed a ceRNA network for EC and found that LINC-FAM138B inhibits the proliferation,migration,invasion,and promotes the apoptosis of HEC1B cells.

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Memo:
海南省三亚市重点科技项目（编号：三科信字［2009］62号）
Last Update: 1900-01-01