|Table of Contents|

LncRNA IQCH-AS1 inhibits proliferation of progesterone-resistant endometrial carcinoma cells by regulating miR-494-3p/ARID1A axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3335-3341
Research Field:
Publishing date:

Info

Title:
LncRNA IQCH-AS1 inhibits proliferation of progesterone-resistant endometrial carcinoma cells by regulating miR-494-3p/ARID1A axis
Author(s):
QIANG Xian; OUYANG Jun; WANG Haizhen; GE Sumei
Department of Pathology,Changzhou Maternal and Child Health Care Hospital,Jiangsu Changzhou 213003,China.
Keywords:
LncRNA IQCH-AS1; miR-494-3p; ARID1A; progesterone; drug resistance; endometrial carcinoma
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.18.001
Abstract:
Objective:To investigate the impacts of LncRNA IQCH-AS1 on the proliferation and apoptosis of progesterone-resistant endometrial carcinoma (EC) cells by regulating miR-494-3p/ARID1A axis.Methods:Ishikawa cells were cultured in vitro and the EC progesterone resistant cell line Ishikawa/MPA was constructed.MTT method was applied to verify the drug resistance of Ishikawa/MPA cells.The expression of progesterone receptor (PR),LncRNA IQCH-AS1,miR-494-3p and ARID1A mRNA in Ishikawa cells and Ishikawa/MPA cells was detected by qRT-PCR.The double luciferase reporter gene experiment and RNA pull-down experiment were applied to verify the targeting relationship between LncRNA IQCH-AS1,miR-494-3p and ARID1A in Ishikawa/MPA cells.Ishikawa/MPA cells were grouped into Ad-NC group,Ad-IQCH-AS1 group,Ad-IQCH-AS1+miR-NC group and Ad-IQCH-AS1+miR-494-3p group.The expression of LncRNA IQCH-AS1,miR-494-3p,ARID1A mRNA and PR mRNA was detected by qRT-PCR.The cell proliferation activity was detected by MTT.The apoptosis rate was detected by flow cytometry.Western blot was applied to detect the expression of ARID1A,PR,CyclinD1,CDK4,cleaned-capase-3,and cleaned-PARP proteins.The effect of LncRNA IQCH-AS1 on the growth and MPA resistance of Ishikawa/MPA cells transplanted tumor in nude mice was observed by tumorigenesis experiment in nude mice.Results:The drug resistance index of Ishikawa/MPA cells constructed in this study was 4.33.The expression of PR mRNA,LncRNA IQCH-AS1 and ARID1A mRNA in Ishikawa/MPA cells was low,while the expression of miR-494-3p was high (P＜0.05).It had been verified that miR-494-3p in Ishikawa/MPA cells had a targeted relationship with LncRNA IQCH-AS1 and ARID1A.Overexpression of LncRNA IQCH-AS1 in Ishikawa/MPA cells could down-regulate the expression of miR-494-3p,and up-regulate the expression of ARID1A,PR mRNA and protein,reduce the cell survival rate and the expression of CyclinD1,CDK4 proteins,and increase the cell apoptosis rate and the expression of cleaved-capase-3,cleaved-PARP proteins (P＜0.05).The overexpression of miR-494-3p was able to attenuate the effect of overexpression of LncRNA IQCH-AS1 on the expression of ARID1A,PR,cell proliferation and apoptosis.In addition,overexpression of LncRNA IQCH-AS1 was able to inhibit the growth of transplanted tumor of Ishikawa/MPA cells in nude mice and reduce MPA resistance.Conclusion:LncRNA IQCH-AS1 regulates miR-494-3p/ARID1A axis to inhibit the proliferation of progesterone-resistant EC cells and induce their apoptosis.

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江苏省常州市卫生健康青苗人才培养（编号：CZQM2020102）
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