|Table of Contents|

Sophoridine mediates apoptosis of prostate cancer PC3 cells through JNK/MAPK signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 17
Page:
3170-3175
Research Field:
Publishing date:

Info

Title:
Sophoridine mediates apoptosis of prostate cancer PC3 cells through JNK/MAPK signaling pathway
Author(s):
ZHU Baoan1; LI Xianjia1; ZHANG Fuhua2
1.Department of Biochemistry；2.Department of Traditional Chinese Medicine，Luohe Medical College，Henan Luohe 462002，China.
Keywords:
sophoridine; prostate cancer; cell apoptosis; JNK/MAPK signaling pathway
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2023.17.006
Abstract:
Objective:To investigate the effect of sophoridine on apoptosis of prostate cancer PC3 cells and the possible mechanism of promoting apoptosis.Methods:The inhibitory effect of sophoridine on the proliferation of PC3 cells was analyzed by MTT method.Trypan blue staining assay was used to detect the effect of sophoridine on the growth and proliferation of PC3 cells.Hoechst 33342 staining and flow cytometry were used to detect the effect of sophoridine on apoptosis of PC3 cells.Western blot detected the effect of sophoridine on the expression of Caspase-3,Bax,Bcl-2,and apoptosis signaling pathway proteins p-JNK,p-p38,p-ERK in PC3 cells.Results:With the increase of sophoridine concentration,the inhibition rate of PC3 cells was gradually increased in a dose-dependent manner,and the proliferation of PC3 cells was significantly inhibited（P<0.05）.The apoptosis rate of sophoridine group was significantly increased,and the expressions of Caspase-3 and Bax apoptosis-promoting proteins were significantly increased,while the expressions of Bcl-2 anti-apoptosis-inhibiting proteins were significantly decreased（P<0.05）.The expression of MAPK signaling pathway protein p-JNK was significantly increased,while the expression of p-p38 and p-ERK had no significant differences（P>0.05）.JNK inhibitor SP600125 reversed the promoting effect of sophoridine on apoptosis of PC3 cells（P<0.05）.Sophoridine could reduce tumor volume and mass in nude mice（P<0.05）.Conclusion:Sophoridine can inhibit the proliferation and promote the apoptosis of PC3 cells in prostate cancer,and has good antitumor activity.It may mediate apoptosis of prostate cancer PC3 cells through JNK/MAPK signaling pathway.

References:

［1］SEKHOACHA M,RIET K,MOTLOUNG P,et al.Prostate cancer review:Genetics,diagnosis,treatment options,and alternative approaches［J］.Molecules,2022,27(17):5730.
［2］邓姣，马金鹏，杨德林.前列腺癌早期诊断的研究进展［J］.现代肿瘤医学，2022，30(5)：903-907. DENG J,MA JP,YANG DL.Research progress of early diagnosis of prostate cancer［J］.Modern Oncology,2022,30(5):903-907.
［3］FENG RM,ZONG YN,CAO SM,et al.Current cancer situation in China:good or bad news from the 2018 global cancer statistics［J］.Cancer Commun(Lond),2019,39(1):22.
［4］HATANO K,TOHYAMA N,KODAMA T,et al.Current status of intensity-modulated radiation therapy for prostate cancer:History,clinical results and future directions［J］.Int J Urol，2019,26(8):775-784.
［5］陈宣谕，洪含霞.老年前列腺癌患者术后发生生化复发的影响因素［J］.中国老年学杂志，2022，42(22)：5463-5466. CHEN XY,HONG HX.Factors influencing the incidence of postoperative biochemical recurrence in elderly patients with prostate cancer［J］.Chinese Journal of Gerontology,2022,42(22)：5463-5466.
［6］CHEN XD,HUA XY,KONG XM,et al.Sophoridine inhibits proliferation and promotes apoptosis of MKN45 cells in human gastric cancer［J］.Acta Physiologica Sinica,2018,70(4):391-396.
［7］UR RASHID H,RASOOL S,ALI Y,et al.Anti-cancer potential of sophoridine and its derivatives:Recent progress and future perspectives［J］.Bioorg Chem,2020,99:103863.
［8］张明发，沈雅琴.槐定碱的抗肿瘤药理作用研究进展［J］.药物评价研究，2021，44(2)：452-460. ZHANG MF,SHEN YQ.Advances in the study of antitumor pharmacological effects of Sophoridine［J］.Journal of Drug Evaluation Research,2021,44(2):452-460.
［9］ZHUANG H,DAI X,ZHANG X,et al.Sophoridine suppresses macrophage-mediated immunosuppression through TLR4/IRF3 pathway and subsequently upregulates CD8+T cytotoxic function against gastric cancer［J］.Biomed Pharmacother,2020,121:109636.
［10］ZHAO B,HUI X,ZENG H,et al.Sophoridine inhibits the tumour growth of non-small lung cancer by inducing macrophages M1 polarisation via MAPK-mediated inflammatory pathway［J］.Front Oncol,2021,24(11):634851.
［11］任丽平，李先佳，金少举,等.槐定碱通过PI3K/Akt/mTOR信号通路介导自噬抑制胰腺癌细胞增殖［J］.现代预防医学,2021,48(6):1084-1088. REN LP,LI XJ,JIN SJ,et al.Sophoridine inhibits the proliferation of pancreatic cancer cells by inducing autophagy through PI3K/Akt/mTOR signaling pathway［J］.Modern Preventive Medicine,2021,48(6):1084-1088.
［12］MORDECAI J,ULLAH S,AHMAD I.Sulforaphane and its protective role in prostate cancer:A mechanistic approach［J］.Int J Mol Sci,2023,24(8):6979.
［13］LIU J,DONG L,ZHU Y,et al.Prostate cancer treatment-China's perspective［J］.Cancer Lett,2022,550:215927.
［14］HENTZEN NB,MOGAKI R,OTAKE S,et al.Intracellular photoactivation of Caspase-3 by molecular glues for spatiotemporal apoptosis induction［J］.J Am Chem Soc,2020,142(18)：8080-8084.
［15］CHEN J,QIN P,TAO Z,et al.Anticancer activity of methyl protodioscin against prostate cancer by modulation of cholesterol-associated MAPK signaling pathway via FOXO1 induction［J］.Biol Pharm Bull,2023,46(4):574-585.
［16］STEINER I,FLORES-TELLEZ TDNJ,MEVEL R,et al.Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells［J］.Cell Rep,2023,42(4):112377.

Memo

Memo:
河南省科技攻关项目（社会发展领域）(编号：172102310214）；漯河医学高等专科学校科技创新重点项目（编号：2022KJZD17）
Last Update: 2023-07-31