|Table of Contents|

LINC00284 promotes the proliferation,migration and invasion of acute T-lymphoblastic leukemia cells through the miR-3127/STAT3 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 17
Page:
3159-3165
Research Field:
Publishing date:

Info

Title:
LINC00284 promotes the proliferation,migration and invasion of acute T-lymphoblastic leukemia cells through the miR-3127/STAT3 axis
Author(s):
ZHANG Huan1; WANG Yuejiao2; WU Bin1
1.Department of Hematology;2.Department of Rheumatology and Immunology,Shengjing Hospital Affiliated to China Medical University，Liaoning Shenyang 110022,China.
Keywords:
LINC00284; miR-3127; STAT3; T-cell acute lymphoblastic leukemia; biological behaviors
PACS:
R733
DOI:
10.3969/j.issn.1672-4992.2023.17.004
Abstract:
Objective：To explore the regulatory role and potential mechanism of LINC00284 in the progression of T-cell acute lymphoblastic leukemia(T-ALL).Methods：The expression of LINC00284,miR-3127 and STAT3 in T-ALL cell lines were detected by RT-qPCR.Plasmid vectors that interfere with the expression of LINC00284,miR-3127 or STAT3 were transfected into MOLT-4 cells.Cell proliferation and apoptosis were measured by MTT and flow cytometry,and cell migration and invasion were measured by Transwell.The targeting relationship between miR-3127 and LINC00284 or STAT3 was verified through bioinformatics website analysis and dual luciferase reporter gene testing.Results：In T-ALL cell lines,the expression of LINC00284 and STAT3 were upregulated,while the expression of miR-3127 was downregulated.Downregulation of LINC00284 or upregulation of miR-3127 significantly inhibits the proliferation,migration,and invasion of T-ALL cells,promoting cell apoptosis.LINC00284 acts as a molecular sponge for miR-3127,which targets and negatively regulates STAT3 expression.Upregulation of STAT3 can reverse the inhibitory effect of downregulation of LINC00284 on the proliferation,migration,invasion,and promotion of apoptosis of T-ALL cells.Conclusion：These findings suggest that LINC00284 promotes the proliferation,migration,invasion,and inhibition of apoptosis of T-ALL cells by regulating the miR-3127/STAT3 axis,which may provide a new target for T-ALL treatment.

References:

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Memo

Memo:
National Natural Science Foundation of China（No.82101898）;国家自然科学基金（编号：82101898）；辽宁省民生科技计划联合计划项目（编号：2021JH2/10300092）
Last Update: 2023-07-31