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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 17

Page:

3153-3159

Research Field:

Publishing date:

Info

Title:

circFTO promotes malignant progression of nasopharyngeal carcinoma by targeting the JAK2/STAT3 signaling pathway

Author(s):

YUAN Tailei¹; 2; LI Wei³; JIN Mingming²; TIAN Kaisai¹; 2; WANG Yuhan²; CHEN Kaibin²; GAN Renfeng²; CHI Yongbin⁴

1.Postgraduate Training Base in Shanghai Pudong Gongli Hospital,Ningxia Medical University,Shanghai 200135,China£»2.Shanghai Key Laboratory of Molecular Imaging,Shanghai University of Medicine & Health Sciences,Shanghai 201318,China;3.School of Public Health,Nanjing Medical University,Jiangsu Nanjing 211166,China£»4.Shanghai Health Commission Key Lab of Artificial Intelligence(AI)-Based Management of Inflammation and Chronic Diseases,Shanghai Pudong Gongli Hospital,Shanghai 200135,China.

Keywords:

circFTO; nasopharyngeal carcinoma; JAK2/STAT3 signaling pathway; epithelial-mesenchymal transition

PACS:

R739.6

DOI:

10.3969/j.issn.1672-4992.2023.17.003

Abstract:

Objective:To investigate the expression of circFTO in nasopharyngeal carcinoma(NPC) and its mechanism of regulating the malignant progression of nasopharyngeal carcinoma.Methods:The expression of circFTO and subcellular localization of circFTO in nasopharyngeal carcinoma cancer tissues and paracancer tissues were detected by fluorescence in situ hybridization(FISH).The expression of circFTO in nasopharyngeal carcinoma cell lines was identified using RT-qPCR.At the same time,circFTO interference vector was constructed to transfect nasopharyngeal carcinoma cells.Flow cytometry,EDU assay,CCK-8 and Western blot were used to explore the function of circFTO in nasopharyngeal carcinoma.Results:circFTO expression was upregulated in nasopharyngeal carcinoma and circFTO was mainly localized in the cytoplasm(P£¼0.05).Down-regulation of circFTO significantly inhibited the proliferation and migration of nasopharyngeal carcinoma cells,and increased apoptosis and apoptosis-related protein expression of nasopharyngeal carcinoma cells(P£¼0.05).Silencing circFTO inhibited the cell cycle of nasopharyngeal carcinoma cells in the G0/G1 phase(P£¼0.000 1).In addition,mechanism studies had shown that circFTO silencing can significantly down-regulate the expression of pJAK2 and pSTAT3 in nasopharyngeal carcinoma cells,and inhibit the epithelial-mesenchymal transition(EMT)(P£¼0.05).Conclusion:circFTO is highly expressed in nasopharyngeal carcinoma tissues and nasopharyngeal carcinoma cells,and may regulate epithelial-mesenchymal transformation to regulate malignant progression of nasopharyngeal carcinoma by regulating JAK2/STAT3 signaling pathway.

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Last Update: 2023-07-31