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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 16

Page:

2983-2988

Research Field:

Publishing date:

Info

Title:

lncRNA POLG-DT is highly expressed in the tissues of patients with non-small cell lung cancer resistant to neoadjuvant chemotherapy and promotes the proliferation,migration and invasion of cancer cells

Author(s):

WU Yonghui; WU Wenjie; FU Wenfan; JIANG Zeyong; DAI Lu; ZHAO Jian; YIN Jun

Department of Thoracic Surgery，Affiliated Cancer Hospital and Institute of Guangzhou Medical University，Guangdong Guangzhou 510000，China.

Keywords:

long non-coding RNA POLG-DT; neoadjuvant chemotherapy; resistance to chemotherapy; NSCLC

PACS:

R734.2

DOI:

10.3969/j.issn.1672-4992.2023.16.007

Abstract:

Objective:To explore the expression of lncRNA POLG-DT in the tissues of patients with non-small cell lung cancer resistant to neoadjuvant chemotherapy,and observe its influence on A549 and NCI-H1299 cell functions.Methods:Postoperative specimens of 7 patients with non-small cell lung cancer were collected and divided into neoadjuvant sensitive group and neoadjuvant drug resistant group according to clinical efficacy.Transcriptome sequencing was used to detect the expression level of mRNA in two groups of patients,and the differentially expressed lncRNA was screened to verify the expression of POLG-DT in non-small cell lung cancer cell lines.The expression level of POLG-DT in A549 and NCI-H1299 cells was down-regulated by transfection of si-RNA.The proliferation ability of cells was detected by CCK8 experiment and colony formation experiment.The migration and invasion ability of A549 and NCI-H1299 cells were detected by Transwell migration experiment and scratch experiment.Results:By sequencing the transcriptome of 7 patients, it was found that the expression of POLG-DT was significantly increased in the neoadjuvant chemotherapy resistant group, with statistical significance（P＜0.05）.Compared with the normal lung epithelial cell line BEAS2B, the expression of POLG-DT in A549 and NCI-H1299 cells increased with statistical significance（P＜0.05）.Down-regulating the expression of POLG-DT in lung cancer cells, the proliferation, migration and invasion ability of A549 and NCI-H1299 cells decreased.Flow cytometry showed that inhibiting the expression of POLG-DT gene could significantly increase the ratio of G0/G1 phase（P＜0.01），and decrease the ratio of S phase（P＜0.05）.Conclusion:POLG-DT can promote the proliferation, migration and invasion of non-small cell lung cancer cells, promote tumor development and make patients resistant to neoadjuvant chemotherapy.

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Memo

Memo:

广东省广州市科技局基础研究计划项目(编号：2060206)

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