|Table of Contents|

Analysis of the effect of PECAM1 on the migration and invasion of gastrointestinal stromal tumor cells based on single-cell transcriptome sequencing data

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
2961-2965
Research Field:
Publishing date:

Info

Title:
Analysis of the effect of PECAM1 on the migration and invasion of gastrointestinal stromal tumor cells based on single-cell transcriptome sequencing data
Author(s):
HU Wu1; WEN Yiqiao2; QIN Jialan1; ZHAO Ying3; ZHANG Tianbiao1
1.Department of Biochemistry and Molecular Biology,School of Life Sciences,China Medical University,Liaoning Shenyang 110122,China;2.The Third Division of Clinical Medicine,China Medical University,Liaoning Shenyang 110122,China;3.Department of Gastrointestinal Nutrition,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China.
Keywords:
gastrointestinal stromal tumor; single-cell transcriptome sequencing; PECAM1; migration and invasion
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.16.003
Abstract:
Objective:To investigate the effect of platelet and endothelial cell adhesion molecule 1(PECAM1) expression on migration and invasion ability in gastrointestinal stromal tumor（GIST) cells.Methods:The target gene was screened based on GIST single-cell transcriptome sequencing data by R language.Cells were transfected with siRNA to establish a low expression group.The effect of PECAM1 on cell migration and invasion was analyzed by wound healing assay and Transwell assay.Results:Six of eighteen GIST cell clusters were defined as non-immune cell populations based on the positive expression of PTPRC.According to the different expression levels of cancer stem cell markers CD44,CD34 and ABCB1,the non-immune cell populations were further divided into four cancer stem cell subpopulations.PECAM1 gene was screened by KEGG analysis with differential expression genes in four cancer stem cell subpopulations.Further gene set enrichment analysis showed that PECAM1 was associated with cell migration signaling pathway.Cell experiments showed that cell migration and invasion ability were significantly decreased after inhibiting expression level of PECAM1,compared to the negative control group（P＜0.05).Conclusion:PECAM1 positively regulates cell migration and invasion in GIST cells.

References:

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Memo

Memo:
National Natural Science Foundation of China（No.61973316）;国家自然科学基金面上项目（编号：61973316）；国家自然科学基金青年基金（编号：61703435）
Last Update: 1900-01-01