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lncRNA ZFAS1 expression in lung cancer and its effects on biological function of lung cancer cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2600-2605
Research Field:
Publishing date:

Info

Title:
lncRNA ZFAS1 expression in lung cancer and its effects on biological function of lung cancer cells and its mechanism
Author(s):
WANG Yun; SONG Shan; PENG Fei; HU Wenxia
Department of Respiration,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China.
Keywords:
lung cancer; ZFAS1; siRNA; NCI-H460; NCI-H1299
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.14.007
Abstract:
Objective:To explore the expression of lncRNA ZFAS1 in lung cancer and its effects on biological function of lung cancer cells,and explore its possible molecular mechanisms by constructing the lung cancer xenograft model of nude mice.Methods:The mRNA expression level of ZFAS1 in lung cancer tissues and cell lines was detected by quantitative real time polymerase chain reaction (qRT-PCR).Positive indicator of ZFAS1 in lung cancer tissues was detected by fluorescence in situ hybridization (FISH).The sensitivity and specificity of ZFAS1 in serum for diagnosis of lung cancer were determined by receiver operating characteristic (ROC) curve.Effects of up-regulating/down-regulating ZFAS1 on NCI-H1299 and NCI-H460 cell invasion,migration and apoptosis were detected by Transwell,wound-healing assay and Tunel assay.Subcutaneous tumor growth of nude mice was detected.Cell proliferation of nude mice in vivo was detected by Ki67 and PCNA immunofluorescence as well as PCNA immunohistochemistry.Results:ZFAS1 mRNA expression was up-regulated in both lung cancer tissues and cell lines.ROC curve showed that when the relative expression level of ZFAS1 was 0.84,its sensitivity and specificity were 86.7% and 76.7%,respectively.Overexpression of ZFAS1 could promote NCI-H1299 cell invasion,migration and inhibit cell apoptosis,while siZFAS1 could inhibit NCI-H460 cell invasion,migration and promote cell apoptosis.ZFAS1 promoted subcutaneous tumor growth of nude mice.Positive indicator of ZFAS1 in subcutaneous lung cancer tissue of nude mice was significantly increased.Up-regulating ZFAS1 could promote cell proliferation of nude mice in vivo,while down-regulating ZFAS1 could produce inhibitory effects.Conclusion:ZFAS1 could facilitate invasion,migration and inhibit apoptosis in NCI-H460 and NCI-H1299 cells,and promote subcutaneous tumor growth and cell proliferation in vivo in nude mice,while siZFAS1 could produce opposite effects.Thus,ZFAS1 could serve as a new promising therapeutic target for early diagnosis and treatment of lung cancer.

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河北省医学科学研究课题计划(编号：20221317)
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