|Table of Contents|

miR-573 promotes epithelial-mesenchymal transition of lung cancer cells by regulating Netrin-4

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2228-2234
Research Field:
Publishing date:

Info

Title:
miR-573 promotes epithelial-mesenchymal transition of lung cancer cells by regulating Netrin-4
Author(s):
XIAO Lei; XU Danyuan; DUAN Linli; HE Mengzhang
Department of Respiratory Medicine,Panyu District,the Second Affiliated Hospital of Guangzhou Medical University,Guangdong Guangzhou 510140,China.
Keywords:
miR-573; Netrin-4; lung cancer; epithelial-mesenchymal transition
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.12.010
Abstract:
Objective:To investigate the effect of miR-573 on the epithelial-mesenchymal transition(EMT) of lung cancer cells by regulating Netrin-4 (NTN4).Methods:Real-time fluorescent quantitative PCR(qRT-PCR) was used to detect the levels of miR-573 and NTN4 mRNA in human lung cancer tissues and cell lines.Lung cancer A549 cells were transfected with Lipofectamine 2000 transfection reagent and grouped into control group(not transfected),NC inhibitor group(transfected with NC inhibitor),miR-573 inhibitor group (transfected with miR-573 inhibitor),miR-573 inhibitor+si-NC group(transfected with miR-573 inhibitor and si-NC) and miR-573 inhibitor+si-NTN4 group(transfected with miR-573 inhibitor and si-NTN4).qRT-PCR was used to detect the expression level of miR-573.Scratch method was used to detect the migration ability.Transwell cell method was used to detect the invasion and migration abilities.Western blot was used to detect the expression levels of NTN4,E-cadherin,N-cadherin and Vimentin.Dual luciferase experiment was used to verify the targeting relationship between miR-573 and NTN4.Results:The expression of miR-573 was significantly increased and the expression of NTN4 mRNA was significantly decreased in lung cancer tissues and cell lines (P<0.05).The A549 cells with the highest relative expression of miR-573 were selected for transfection experiments.Compared with the control group or NC inhibitor group,the relative expression of miR-573 and the protein levels of N-cadherin and Vimentin in the A549 cells of the miR-573 inhibitor group were significantly decreased.The scratch healing rate was significantly reduced.The numbers of migrating and invading cells were significantly reduced,and the level of E-cadherin protein was significantly increased (P<0.05).miR-573 negatively regulated NTN4 expression (P<0.05).Transfection of si-NTN4 could reverse the inhibitory effects of miR-573 inhibitor on migration,invasion and EMT of A549 cells (P<0.05).Conclusion:miR-573 targets and negatively regulates the expression of NTN4 and promotes the migration,invasion and EMT of lung cancer cells.

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