|Table of Contents|

Ursolic acid reverses epithelial-mesenchymal transition and angiogenesis in lung cancer A549 cells through HGF/c-met pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2203-2208
Research Field:
Publishing date:

Info

Title:
Ursolic acid reverses epithelial-mesenchymal transition and angiogenesis in lung cancer A549 cells through HGF/c-met pathway
Author(s):
LIN Chen; LI Shuang; FENG Tao; XU Guixiang
Department of Respiratory and Critical Care Medicine,Shengli Oilfield Central Hospital,Shandong Dongying 257000,China.
Keywords:
ursolic acid; HGF; EMT; angiogenesis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.12.006
Abstract:
Objective:To explore the mechanism of ursolic acid on epithelial-mesenchymal transition and angiogenesis in lung cancer cells induced by hepatocyte growth factor (HGF).Methods:The effect of ursolic acid (0,5,10,20,40,80 μmol/L) on the proliferation of A549 cells were measured by CCK-8 assay.Transwell assay was used to determine the effect of ursolic acid on cell invasion.A549 cells were induced by HGF to establish EMT model,and the morphological changes of cells were observed under inverted microscope.The influence of ursolic acid on the angiogenesis of lung cancer was detected by the tube formation assay.The protein expression levels of E-cadherin,Vimentin and p-met were detected by Western blotting.Results:Ursolic acid significantly inhibited the proliferation of lung cancer A549 cells.IC50 of ursolic acid (24 h) for the A549 cells was 31.75 μmol/L.Ursolic acid significantly inhibited cell invasion and epithelial-mesenchymal transition of lung cancer A549 cells induced by HGF(P<0.01).Moreover,ursolic acid also inhibited HGF-induced angiogenesis of endothelial cells(P<0.01).In addition,molecular mechanism study showed that ursolic acid could inhibit HGF activated HGF/c-met signaling.At the same time,we used the c-met inhibitor SU11274 to further investigate its effects on epithelial-mesenchymal transition and angiogenesis in lung cancer A549 cells,and found that SU11274 had similar effects to ursolic acid.Conclusion:Ursolic acid inhibits proliferation,invasion,epithelial-mesenchymal transition and angiogenesis of lung cancer A549 cells,and the mechanism may be related to HGF/c-met signaling pathway.

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山东省医药卫生科技发展计划项目（编号：202103020493）
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