|Table of Contents|

hsa_circ_0000520 promotes occurrence and metastasis of breast cancer by regulating miR-556-5p/SLC38A2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2190-2196
Research Field:
Publishing date:

Info

Title:
hsa_circ_0000520 promotes occurrence and metastasis of breast cancer by regulating miR-556-5p/SLC38A2
Author(s):
ZHANG Jingchen; LI Xin; LI Jiangtao; LI Haiping; CHEN Yanli; NIU Bing; QI Chuanchuan; YE Beibei
Department of Galactophore,Zhengzhou People's Hospital,Henan Zhengzhou 450053,China.
Keywords:
hsa_circ_0000520; miR-556-5p; solute carrier family 38 member 2; breast cancer
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.12.004
Abstract:
Objective:To explore the mechanism of hsa_circ_0000520 in promoting the occurrence and metastasis of breast cancer.Methods:Dual-luciferase reporter gene experiments and RNA pull-down experiments verified the targeting relationship of miR-556-5p with hsa_circ_0000520 and solute carrier family 38 member 2 (SLC38A2).MCF7 cells were separated into sh-NC group,sh-hsa_circ_0000520 group,sh-hsa_circ_0000520+anti-NC group,and sh-hsa_circ_0000520+anti-miR-556-5p group.qRT-PCR or Western blot were applied to detect the expression levels of hsa_circ_0000520,miR-556-5p and SLC38A2 in cells.MTT assay and plate colony formation assay were used to evaluate cell proliferation.Scratch-healing assay and Transwell assay were used to evaluate cell migration and invasion abilities.The nude mice tumorigenesis experiments were applied to evaluate the regulatory effect of hsa_circ_0000520 on miR-556-5p/SLC38A2 and its effect on the growth of xenografts.Results:It had been verified that miR-556-5p had a targeting relationship with hsa_circ_0000520 and SLC38A2 in MCF7 cells.Compared with the sh-NC group,the sh-hsa_circ_0000520 group could reduce the expression levels of hsa_circ_0000520 and SLC38A2 mRNA and protein,cell viability,number of colonies,wound healing rate,and the numbers of migrating and invasive cells in MCF7 cells (P<0.05),increase the expression level of miR-556-5p(P<0.05).Compared with sh-hsa_circ_0000520+anti-NC group,sh-hsa_circ_0000520+anti-miR-556-5p group could reduce the expression level of miR-556-5p in MCF7 cells (P<0.05),increase the expression levels of SLC38A2 mRNA and protein,cell viability,number of colonies,wound healing rate,and numbers of migrating and invasive cells (P<0.05),however,it had no obvious effect on the expression of hsa_circ_0000520 (P>0.05).The results of tumorigenesis experiments in nude mice showed that knocking down the expression of hsa_circ_0000520 in xenograft tumors could increase the expression level of miR-556-5p and decrease the expression levels of SLC38A2 mRNA and protein,and reduce tumor volume and tumor weight (P<0.05).Conclusion:hsa_circ_0000520 may promote the occurrence and metastasis of breast cancer by targeting miR-556-5p/SLC38A2.

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河南省医学科技攻关计划（联合共建）项目（编号:LHGJ20191074,201303219）
Last Update: 1900-01-01