|Table of Contents|

Expression and functional analysis of long chain non-coding RNA in laryngeal papilloma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 11
Page:
2003-2007
Research Field:
Publishing date:

Info

Title:
Expression and functional analysis of long chain non-coding RNA in laryngeal papilloma
Author(s):
Sarah·Ornbek1; Aigul·Medeulo2; Ayhen·Qukuerhan1
1.Department of Otorhinolaryngology,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China;2.S.D.Asfendiyarov Kazakh National Medical University Aksay Hospital(Children's Hospital),Kazakhstan Almaty 050063.
Keywords:
laryngeal papilloma; long chain non-coding RNA; differentially expressed genes; RNA sequencing
PACS:
R739.65
DOI:
10.3969/j.issn.1672-4992.2023.11.006
Abstract:
Objective:To analyze the differential expression profile of long chain non-coding RNA (lncRNA) in laryngeal papilloma (LP) and predict its target genes,and provide new ideas and targets for the treatment and prevention of laryngeal papilloma.Methods:The RNA sequencing（RNA-seq） technologies was used for the sequencing in laryngeal papilloma and adjacent tissue.The multiples differences fold change (FC) was as a standard for screening differentially expressed genes,and lncRNA cis-regulatory target genes in the range of 10 kb were analyzed and extracted,and their biological functions and participation in signaling pathways were analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG),etc.Results:A total of 227 differentially expressed lncRNAs were selected (Log2FC≥2 or ≤0.5,P<0.05),of which 76 (33%) were highly expressed up-regulated genes and 151 (67%) were down-regulated genes.There were 44 lncRNA cis-regulatory target genes that met the positional requirements and co-expression relationships,and the results of enrichment analysis into GO and KEGG pathways showed that differentially expressed genes GO BP were significantly enriched in signaling and KEGG pathway were significantly enriched in MAPK signaling pathway and PI3K-Akt signaling pathway.Conclusion:Differential expression of lncRNA and its target genes as well as related signaling pathways may be closely associated with laryngeal papilloma recurrence and its carcinogenesis.

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Memo

Memo:
哈萨克斯坦儿童复发性呼吸道乳头状瘤病临床诊治平台建设（编号：2020E01055）
Last Update: 2023-04-28