|Table of Contents|

The mechanism of miR-145-5p regulating invasion and migration of colorectal cancer SW620 cells by targeting TAGLN2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 11
Page:
1986-1991
Research Field:
Publishing date:

Info

Title:
The mechanism of miR-145-5p regulating invasion and migration of colorectal cancer SW620 cells by targeting TAGLN2
Author(s):
ZHENG Liuchang1; WANG Rong1; WEN Dongpeng2; ZHAO Weifeng3
1.Department of Clinical Laboratory;2.Gastrointestinal Surgery;3.Department of Medical Oncology,Henan Provincial People's Hospital,Henan Zhengzhou 450003,China.
Keywords:
colorectal cancer; miR-145-5p; TAGLN2; invasion; migration
PACS:
R735.3+4
DOI:
10.3969/j.issn.1672-4992.2023.11.003
Abstract:
Objective:To explore the expression of miR-145-5p in colorectal cancer (CRC),and to investigate the effect of miR-145-5p on the invasion and migration of CRC cells by targeting TAGLN2.Methods:Quantitative real-time PCR (qPCR) was used to detect the expression of miR-145-5p and TAGLN2 mRNA in 48 cases of CRC tissues and corresponding adjacent tissues,CRC cell lines (HCT8,SW620,HCT116,HT-29) and human normal colorectal mucosal cells FHC.The SW620 cells were divided into blank control group,miR-145-5p mimics group,mimics-NC group,pcDNA3.1-TAGLN2 group,pcDNA3.1-Vector group and miR-145-5p mimics+pcDNA3.1-TAGLN2 group.The expression of miR-145-5p and TAGLN2 mRNA in SW620 cells was detected by qPCR.Transwell assay was used to detect the invasion and migration of SW620 cells.The expression of TAGLN2 and EMT-related proteins in SW620 cells was detected by Western blot.The targeting relationship between miR-145-5p and TAGLN2 was detected by dual luciferase reporter assay.Results:The expression of miR-145-5p in CRC tissues was significantly lower than that in adjacent tissues (P<0.05),which was correlated with TNM stage and lymphatic metastasis in CRC patients (all P<0.05).The expression of TAGLN2 in CRC tissues was significantly higher than that in adjacent tissues,and was negatively correlated with the expression of miR-145-5p (P<0.05).The expression of miR-145-5p and TAGLN2 in CRC cell lines（HCT8,SW620,HCT116 and HT-29）were significantly lower or higher than that in FHC cells(all P<0.05).Transfection with miR-145-5p mimics could reduce the invasion and migration of SW620 cells.miR-145-5p targeted the expression of TAGLN2.The up-regulation of TAGLN2 can promote the invasion and migration of SW620 cells.After co-transfection with miR-145-5p mimics,the expression of TAGLN2,Vimentin and N-cadherin was decreased,but the expression of E-cadherin was increased.The positive effects of TAGLN2 overexpression on invasion and migration of SW620 cells were significantly inhibited.Conclusion:The low expression of miR-145-5p in CRC was closely related to TNM stage and lymphatic metastasis of CRC patients.miR-145-5p inhibit the invasion and migration of colorectal cancer cells by targeting TAGLN2.

References:

［1］SHAUKAT A,KAHI CJ,BURKE CA,et al.ACG clinical guidelines:Colorectal cancer screening 2021［J］.Am J Gastroenterol,2021,116(3):458-479.
［2］曹毛毛,陈万青.GLOBOCAN 2020全球癌症统计数据解读［J］.中国医学前沿杂志（电子版）,2021,13(3):63-69. CAO MM,CHEN WQ.Interpretation on the global cancer statistics of GLOBOCAN 2020［J］.Chinese Journal of the Frontiers of Medical Science(Electronic Version),2021,13(3):63-69.
［3］吴春晓,顾凯,龚杨明,等.2015年中国结直肠癌发病和死亡情况分析［J］.中国癌症杂志,2020,30(4):241-245. WU CX,GU K,GONG YM,et al.Analysis of incidence and mortality of colorectal cancer in China,2015［J］.China Oncology,2020,30(4):241-245.
［4］PENG P,LUAN Y,SUN P,et al.Prognostic factors in stage IV colorectal cancer patients with resection of liver and/or pulmonary metastases:A population-based cohort study ［J］.Front Oncol,2022,12:850937.
［5］DAVEY MG,FEENEY G,ANNUK H,et al.MicroRNA expression profiling predicts nodal status and disease recurrence in patients treated with curative intent for colorectal cancer ［J］.Cancers (Basel),2022,14(9):2109.
［6］MORISHITA A,SHIRAKAMI Y,OKUMURA T,et al.MicroRNA as a biomarker in gastroenterological cancers ［J］.Int J Mol Sci,2022,23(9):4701.
［7］KOLECKOVA M,EHRMANN J,BOUCHAL J,et al.Epithelial to mesenchymal transition and microRNA expression are associated with spindle and apocrine cell morphology in triple-negative breast cancer ［J］.Sci Rep,2021,11(1):5145.
［8］武磊,周文勤,袁琳楠,等.miR-145-5p靶向ARK5调控人上皮性卵巢癌细胞增殖凋亡的作用［J］.中国医学科学院学报,2021,43(5):669-676. WU L,ZHOU WQ,YUAN LN,et al.Role of miR-145-5p targeting ARK5 in regulating the proliferation and apoptosis of human epithelial ovarian cancer cells ［J］.Acta Academiae Medicinae Sinicae,2021,43(5):669-676.
［9］JI S,SHI Y,YANG L,et al.miR-145-5p inhibits neuroendocrine differentiation and tumor growth by regulating the SOX11/MYCN axis in prostate cancer ［J］.Front Genet,2022,13:790621.
［10］NIU Y,ZHANG J,TONG Y,et al.miR-145-5p restrained cell growth,invasion,migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway ［J］.Int J Biochem Cell Biol,2019,117:105641.
［11］ZENG C,ZHANG H.Mechanisms of transgelin-2 in tumorigenesis［J］.Discov Med,2021,32(165):23-28.
［12］ZHANG B,GAO S,BAO Z,et al.MicroRNA-656-3p inhibits colorectal cancer cell migration,invasion,and chemo-resistance by targeting sphingosine-1-phosphate phosphatase 1［J］.Bioengineered,2022,13(2):3810-3826.
［13］PIAWAH S,VENOOK AP.Targeted therapy for colorectal cancer metastases:A review of current methods of molecularly targeted therapy and the use of tumor biomarkers in the treatment of metastatic colorectal cancer ［J］.Cancer,2019,125(23):4139-4147.
［14］KADKHODA S,GHAFOURI-FARD S.Function of miRNA-145-5p in the pathogenesis of human disorders ［J］.Pathol Res Pract,2022,231:153780.
［15］张雨薇，张馨月，白立川，等.ceRNA调控miR-145-5p介导肿瘤发生发展的研究进展 ［J］.现代肿瘤医学，2020,28（10）：1766-1769. ZHANG YW,ZHANG XY,BAI LC,et al.The research progress of ceRNA regulating miR-145-5p in cancer［J］.Modern Oncology,2020,28（10）：1766-1769.
［16］ZHANG Q,CHENG Z,SHI L,et al.miR-145-5p inhibits the proliferation of glioma stem cells by targeting translationally controlled tumor protein ［J］.J Cancer,2022,13(5):1490-1500.
［17］WANG T,DONG L,SUN J,et al.miR-145-5p:A potential biomarker in predicting gleason upgrading of prostate biopsy samples scored 3+3=6 ［J］.Cancer Manag Res,2021,13:9095-9106.
［18］WANG S,WANG T,GU P.microRNA-145-5p inhibits migration,invasion,and metastasis in hepatocellular carcinoma by inhibiting ARF6［J］.Cancer Manag Res,2021,13:3473-3484.
［19］马成斌,刘平,刘彧,等.子宫内膜样腺癌组织中差异表达miRNA筛选及其与患者预后高危因素的关系［J］.山东医药,2021,26(1):29-34. MA CB,LIU P,LIU Y,et al.Screening of differentially expressed miRNAs in endometrial endometrioid adenocarcinoma tissues and their relationships with high-risk factors for prognosis of patients ［J］.Shandong Medical Journal,2021,26(1):29-34.
［20］DING R,LI G,YAO Y,et al.Transgelin-2 interacts with CD44 to regulate Notch1 signaling pathway and participates in colorectal cancer proliferation and migration ［J］.J Physiol Biochem,2022,78(1):99-108.
［21］ZHAO Z,LU L,LI W.TAGLN2 promotes the proliferation,invasion,migration and epithelial-mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2 ［J］.Oncol Lett,2021,22(4):737.
［22］HUANG Y,ZHANG H,WANG L,et al.MiR-613 inhibits the proliferation,migration,and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2［J］.Cancer Cell Int,2021,21(1):494.
［23］ZHAO F,ZHOU LH,GE YZ,et al.MicroRNA-133b suppresses bladder cancer malignancy by targeting TAGLN2-mediated cell cycle ［J］.J Cell Physiol,2019,234(4):4910-4923.

Memo

Memo:
National Natural Science Foundation of China(No.82002210）；国家自然科学基金资助项目（编号：82002210）
Last Update: 2023-04-28