|Table of Contents|

Cisplatin combinated with Rosmarinic acid inhibits proliferation and induces apoptosis of Huh7 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1821-1825
Research Field:
Publishing date:

Info

Title:
Cisplatin combinated with Rosmarinic acid inhibits proliferation and induces apoptosis of Huh7 cells
Author(s):
TAN Ying1; 2; LI Xuejing1; 2; LIU Bihan2; LIU Wenjie2; WU Xinyu2; ZHANG Lucheng2; WANG Feng3; YUAN Jianhui1; 2
1.Department of Physiology,School of Basic Medical Sciences,Guangxi Medical University,Guangxi Nanning 530021,China;2.Research Center for Biomedical Photonics,Institute of Life Science,Guangxi Medical University,Guangxi Nanning 530021,China;3.Key Laboratory of Biological Molecular Medicine Research (Guangxi Medical University),Education Department of Guangxi Zhuang Autonomous Region,Guangxi Nanning 530021,China.
Keywords:
Cisplatin; Rosmarinic acid; Huh7 cells; proliferation; apoptosis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.10.009
Abstract:
Objective:To investigate the effects of Cisplatin (CDDP) combined with Rosmarinic acid (RA) on the proliferation and apoptosis of human primary hepatocellular carcinoma Huh7 hepatoma cells.Methods:The IC50 values after 24 h,48 h and 72 h treatment with CDDP and RA respectively were detected and calculated by CCK-8 cell proliferation assay.Then,drug untreated group (control group),5 μg/mL CDDP group,200 μg/mL RA group,and 5 μg/mL CDDP combined with 200 μg/mL RA group were set.The apoptosis rates of the control group,CDDP group,RA group and combination group were (7.28±0.56)%,(18.23±2.53)%,(26.44±1.99)% and (37.27±0.28)% by flow cytometry,respectively.Finally,the expression levels of Bcl-2,Bax,Cleaved-caspase-3 and Procaspase-3 apoptosis-related proteins were detected by Western blot.Results:RA enhanced the inhibitory ability of CDDP on the proliferation of Huh7 cells and showed a concentration-dependent relationship (P＜0.05),and the apoptosis rate of Huh7 cells was significantly increased in the CDDP combined with RA-treated group (P＜0.05),and the expression levels of the pro-apoptotic proteins Bax and Cleaved-caspase-3 increased after the effect of CDDP combined with RA,while expression levels of Bcl-2 were decreased (P＜0.05).Conclusion:RA can inhibit the proliferation of Huh7 cells and induce cell apoptosis,and has a synergistic effect with Cisplatin.The mechanism may be related to the activation of mitochondrial apoptosis pathway.

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广西自然科学基金(编号：2016GXNSFBA380017)
Last Update: 1900-01-01