|Table of Contents|

Effects of miR-762 on radiosensitivity of endometrial carcinoma Ishikawa cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1805-1810
Research Field:
Publishing date:

Info

Title:
Effects of miR-762 on radiosensitivity of endometrial carcinoma Ishikawa cells and its mechanism
Author(s):
LI Rucai1; PAN Yinglian2; CAI Xingrui2; GUO Lijuan1
1.Department of Radiotherapy;2.Department of Medical Oncology,the First Affiliated Hospital of Hainan Medical University,Hainan Haikou 570102,China.
Keywords:
endometrial carcinoma; miR-762; adenomatosis polyposis coli 2; proliferation; apoptosis; radiosensitivity
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.10.006
Abstract:
Objective:To investigate the expression level of miR-762 in endometrial cancer (EC) patients' cancer tissues and its effects on radiosensitivity of endometrial cancer Ishikawa cells and its mechanism.Methods:qRT-PCR was used to detect the expression levels of miR-762 and adenomatous colonic polyp 2 (APC2) mRNA in 54 EC cancer tissues and adjacent tissues,as well as normal endometrial cells ESC and EC cell lines.Ishikawa cells were treated with different radiation doses,and the expression levels of miR-762 and APC2 mRNA were detected by qRT-PCR.Ishikawa cells were divided into blank control group (blank group),Lv-NC group (infected with miR-762 sponge negative control lentivirus),Lv-miR-762 SP group (infected with miR-762 sponge lentivirus),Lv-miR-762 SP+si-NC group and Lv-miR-762 SP+si-APC2 group.The expression levels of miR-762 and APC2 in cells were detected by qRT-PCR and Western blot.The targeting relationship between miR-762 and APC2 was detected by dual-luciferase reporter gene assay.Ishikawa cells in each group were irradiated with 6 Gy rays.The radiosensitivity of cells was detected by colony formation assay.Cell proliferation activity was detected by CCK-8.Cell apoptosis was detected by flow cytometry.The protein expression levels of CyclinD1,p21,Bcl-2 and Bax were detected by Western blot.Results:miR-762 was highly expressed in EC cancer tissues and cell lines,while APC2 was low expressed.With the increase of radiation dose,the expression level of miR-762 in Ishikawa cells was gradually increased (P＜0.05),and the expression level of APC2 mRNA was gradually decreased (P＜0.05),in a dose-dependent manner.The dual-luciferase reporter gene assay confirmed that APC2 was a target gene of miR-762.Inhibition of miR-762 could significantly down-regulate the expression level of miR-762,up-regulate the expression level of APC2,enhance cell radiosensitivity,inhibit cell proliferation,promote cell apoptosis,and down-regulate CyclinD1 and Bcl-2 protein expression levels in Ishikawa cells,while up-regulate the protein expression levels of p21 and Bax (P＜0.05).Furthermore,interfering with the APC2 gene significantly reversed the promoting effect of inhibiting miR-762 expression on the radiosensitivity of Ishikawa cells.Conclusion:miR-762 was highly expressed in EC cancer tissues and cell lines,and inhibiting its expression could enhance the radiosensitivity of Ishikawa cells by up-regulating the expression of APC2.

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Memo:
海南省卫生健康行业科研项目（编号：20A200431）
Last Update: 1900-01-01