|Table of Contents|

Down-regulation of MYEOV inhibits proliferation,migration and invasion of pancreatic cancer cell line PaTu8988

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1794-1799
Research Field:
Publishing date:

Info

Title:
Down-regulation of MYEOV inhibits proliferation,migration and invasion of pancreatic cancer cell line PaTu8988
Author(s):
CAO Lijun1; ZHANG Xingxing2; ZHANG Yue3; XIE Yaya1; LIU Xianju1; CHEN Hua2; CHEN Jinlian1; 2
1.Anhui University of Science and Technology,Anhui Huainan 232000,China;2.Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus,Shanghai 201499,China;3.Hanzhong Central Hospital,Shaanxi Hanzhong 723000,China.
Keywords:
pancreatic cancer; MYEOV; epithelial-mesenchymal transition; STAT3 signaling pathway
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2023.10.004
Abstract:
Objective:To study the effects of myeloma overexpressed gene (MYEOV) on the proliferation,migration,and invasion of pancreatic cancer cells,as well as the regulation of epithelial-mesenchymal transformation (EMT) related proteins and STAT3 signal pathway-related proteins.Methods:The UCSC Xena database was used to analyze the expression level of MYEOV in pancreatic cancer and normal tissues,and the ICGC database was used to analyze the relationship between MYEOV and the prognosis of pancreatic cancer patients.A lentivirus recombinant PaTu8988 cell line with stable low expression of MYEOV was constructed.Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect MYEOV mRNA and protein expression in the pancreatic cancer cell line.CCK-8,scratch assay,Transwell migration,and invasion assay were used to analyze cell proliferation,migration,and invasion ability.The expressions of E-cadherin,Vimentin,MMP2,MMP9,STAT3,and p-STAT3 were detected by Western blot.Results:Bioinformatics analysis showed that MYEOV was highly expressed in pancreatic cancer tissues and was associated with a poor prognosis of pancreatic cancer.Compared with the control group,the proliferation,migration,and invasion ability of pancreatic cancer cell line PaTu8988 decreased after the down-regulation of MYEOV.Compared with the control group,after the down-regulation of MYEOV,E-cadherin protein expression was increased in the PaTu8988 cell line,and Vimentin,MMP2,MMP9,and p-STAT3 protein expression was down-regulated.Conclusion:The down-regulation of MYEOV expression inhibits the proliferation,migration,and invasion of pancreatic cancer cells,and the mechanism may be related to the inhibition of EMT.The change of STAT3 pathway protein during EMT suggests that the STAT3 pathway may be involved in regulating this phenotype.

References:

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Memo

Memo:
上海市卫生健康委员会青年科研基金（编号：20204Y0181）;上海市奉贤区（社会类）科技发展基金（编号：20211805）
Last Update: 1900-01-01