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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 10

Page:

1780-1784

Research Field:

Publishing date:

Info

Title:

SPON1 promotes migration,invasion and epithelial-mesenchymal transformation of cholangiocarcinoma cells

Author(s):

YANG Liangfang; ZHU Zhiwen; ZHENG Yang; HUANG Haili; HE Huijuan

Department of Hepatobiliary Surgery,the Affiliated Hospital of Guangdong Medical University,Guangdong Zhanjiang 524001,China.

Keywords:

SPON1; cholangiocarcinoma; oncogene; epithelial-mesenchymal transformation

PACS:

R735.8

DOI:

10.3969/j.issn.1672-4992.2023.10.002

Abstract:

Objective:To explore the effect of SPON1 on cell migration,invasion and epithelial-mesenchymal transformation.Methods:The expression of SPON1 was analyzed by bioinformatics and immunohistochemistry.The SPON1 overexpression plasmid,the SPON1 knockdown plasmid,and the negative control plasmid were transferred into the CCA cell line (QBC939,HCCC-9810),and cell migration and invasion capacity were measured by healing assay and Transwell assay.The effect of SPON1 on the epithelial-mesenchymal transformation in CCA cells was measured by Western blot.Results:Both of the bioinformatics analysis results and immunohistochemistry results suggested that SPON1 was highly expressed in CCA (P£¼0.05).Overexpression of SPON1 could effectively enhance the ability of migration and invasion of CCA cells (P£¼0.05).In contrast,silence of SPON1 could inhibit migration and invasion of CCA cells (P£¼0.05).SPON1 promoted epithelial-mesenchymal transformation of CCA cells by upregulating Vimentin and downregulating E-cadherin (P£¼0.05).Conclusion:SPON1 was upregulated in CCA,and that promoted CCA cells migration,invasion,and epithelial-mesenchymal transformation.

References:

£Û1£ÝIZQUIERDO-SANCHEZ L,LAMARCA A,LA CA,et al.Cholangiocarcinoma landscape in Europe:diagnostic,prognostic and therapeutic insights from the ENSCCA Registry£ÛJ£Ý.J Hepatol,2022,76(5):1109-1121.
£Û2£ÝBRINDLEY PJ,BACHINI M,ILYAS SI,et al.Cholangiocarcinoma£ÛJ£Ý.Nat Rev Dis Primers,2021,7(1):65.
£Û3£ÝSCHUBERT D,KLAR A,PARK M,et al.F-spondin promotes nerve precursor differentiation£ÛJ£Ý.J Neurochem,2006,96(2):444-453.
£Û4£ÝKLAR A,BALDASSARE M,JESSELL TM.F-spondin:a gene expressed at high levels in the floor plate encodes a secreted protein that promotes neural cell adhesion and neurite extension£ÛJ£Ý.Cell,1992,69(1):95-110.
£Û5£ÝCLEMITSON JR,DIXON RJ,HAINES S,et al.Genetic dissection of a blood pressure quantitative trait locus on rat chromosome 1 and gene expression analysis identifies SPON1 as a novel candidate hypertension gene£ÛJ£Ý.Circ Res,2007,100(7):992-999.
£Û6£ÝCHANG HP,DONG TH,MA XT,et al.Spondin 1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma£ÛJ£Ý.Biochem Biophys Res Commun,2015,464(1):45-50.
£Û7£ÝDAI W,HUANG HL,HU M,et al.MicroRNA-506 regulates proliferation,migration and invasion in hepatocellular carcinoma by targeting F-spondin 1 (SPON1)£ÛJ£Ý.Am J Cancer Res,2015,5(9):2697-2707.
£Û8£ÝNAGASAWA S,IKEDA K,SHINTANI D,et al.SPON1-TRIM29 identification of a novel oncogenic fusion gene in clinical ovarian cancer that promotes cell and tumor growth and enhances chemoresistance in A2780 cells£ÛJ£Ý.Int J Mol Sci,2022,23(2):689.
£Û9£ÝTAMJIDIFAR R,AKBARI M,TARZI S,et al.Prognostic and diagnostic values of miR-506 and SPON 1 in colorectal cancer with clinicopathological considerations£ÛJ£Ý.J Gastrointest Cancer,2021,52(1):125-129.
£Û10£ÝHUO YM,YANG J,ZHENG JH,et al.Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling£ÛJ£Ý.Cell Prolif,2022,55(5):e13237.
£Û11£ÝWU QB,ZHEN YL,SHI L,et al.EGFR inhibition potentiates FGFR inhibitor therapy and overcomes resistance in FGFR2 fusion-positive cholangiocarcinoma£ÛJ£Ý.Cancer Discov,2022,12(5):1378-1395.
£Û12£ÝSAHAI V,CATALANO PJ,ZALUPSKI MM,et al.Nab-paclitaxel and gemcitabine as first-line treatment of advanced or metastatic cholangiocarcinoma:a phase 2 clinical trial£ÛJ£Ý.JAMA Oncol,2018,4(12):1707-1712.
£Û13£ÝHU HS,XIN N,LIU JX,et al.Characterization of F-spondin in Japanese flounder (Paralichthys olivaceus) and its role in the nervous system development of teleosts£ÛJ£Ý.Gene,2016,575:623-631.
£Û14£ÝLV J,ZHU YZ,JI AL,et al.Mining TCGA database for tumor mutation burden and their clinical significance in bladder cancer£ÛJ£Ý.Biosci Rep,2020,40(4):BSR20194337.
£Û15£ÝVAQUERO J,GUEDJ N,CLAP¦IRON A,et al.Epithelial-mesenchymal transition in cholangiocarcinoma:From clinical evidence to regulatory networks£ÛJ£Ý.J Hepatol,2017,66(2):424-441.
£Û16£ÝLIU B,SONG XD,YAN ZW,et al.MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1£ÛJ£Ý.Oncol Lett,2019,17(1):781-788.

Memo

Memo:

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