|Table of Contents|

Long intergenic non-coding RNA-p21 induces gastric cancer cells apoptosis through YAP expression regulation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1775-1780
Research Field:
Publishing date:

Info

Title:
Long intergenic non-coding RNA-p21 induces gastric cancer cells apoptosis through YAP expression regulation
Author(s):
CHEN Ying1; JIANG Xue1; NI Pengzhi2; ZHENG Lingyan1; YAN Danfang1; YAN Senxiang1
1.Department of Radiation Oncology;2.Department of Thoracic Surgery,the First Affiliated Hospital,Zhejiang University School of Medicine,Zhejiang Hangzhou 310003,China.
Keywords:
gastric cancer; lincRNA-p21; apoptosis; YAP protein
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.10.001
Abstract:
Objective:To explore the role of lincRNA-p21 in gastric cancer (GC) cells apoptosis and the underlying mechanisms.Methods:The siRNA and overexpressing plasmid were transfected to MGC-803 cells down or up regulate lincRNA-p21 expression.The mRNA level of lincRNA-p21 and YAP was assessed by qRT-PCR.Cell apoptosis was quantitated by flow cytometry and TUNEL assay.Apoptosis associated proteins were detected by Western blot.Results:The lincRNA-p21 upregulation induced gastric cancer cells apoptosis,and vice versa.We also found that the expression of YAP mRNA and protein was increased by downregulated lincRNA-p21.Moreover,the anti-apoptotic effect caused by lincRNA-p21 downregulation can be attenuated by the knocking down of YAP expression.Conclusion:lincRNA-p21 could serve as the cells apoptosis inducer in GC by regulation YAP expression,which sheds new light on gastric cancer treatment.

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浙江省自然科学基金资助项目(编号：LQ19H160033)
Last Update: 1900-01-01