|Table of Contents|

Lipopolysaccharide(LPS) stimulates NLRP3/Caspase-1 inflammatory signaling pathway and promotes proliferation and migration of oral squamous cell carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1577-1583
Research Field:
Publishing date:

Info

Title:
Lipopolysaccharide(LPS) stimulates NLRP3/Caspase-1 inflammatory signaling pathway and promotes proliferation and migration of oral squamous cell carcinoma cells
Author(s):
HE Chanjuan; ZHANG Qianyu; ZHANG Weifang; XIAO Qian; LIU Ying
Department of Stomatology,Affiliated Hospital of North Sichuan Medical College,North Sichuan Medical College,Sichuan Nanchong 637000,China.
Keywords:
lipopolysaccharide; NLRP3 inflammasome; Caspase-1; IL-1β
PACS:
R739.8
DOI:
10.3969/j.issn.1672-4992.2023.09.001
Abstract:
Objective：To explore the promotion and migration of oral squmous cell carcinoma cells by LPS stimulation of inflammatory signaling pathway.Methods：The protein and mRNA expressions of NLRP3,Caspase-1 and IL-1β in normal tissues and cancer tissues,normal oral epithelial cell lines(NOK) and OSCC cell lines SCC25 and HSC3 were detected by WB,ELISA and qRT-PCR.It was detected by qRT-PCR,WB,CCK-8 and scratch healing test that the mRNA and protein expression of NLRP3,Caspase-1 and IL-1β before and after LPS stimulation of SCC25 and HSC3 and the effects on cell proliferation and migration biological characteristics.Results：The mRNA and protein expressions of NLRP3,Caspase-1 and IL-1β in OSCC carcinoma tissues were significantly higher than those in adjacent normal tissues,with P values of 0.035 3,0.024,0.036 2,0.035 4,0.041 3 and 0.014 8,respectively.Compared with SCC25 and HSC3 cell lines,the mRNA and protein expression of NLRP3（P＜0.000 1,P＜0.000 1,P＜0.01，P＜0.01),Caspase-1（P＜0.000 1,P=0.000 1,P＜0.01,P＜0.01),IL-1β（P=0.000 3,P=0.001 2,P＜0.001，P＜0.001) was significantly higher than that in NOK cell lines.The mRNA and protein expressions of NLRP3(P＜0.05,P＜0.001),Caspase-1(P＜0.05,P＜0.001) and IL-1β(P＜0.01,P＜0.01) in SCC25 cell lines were significantly increased after 12 h of 1 μg/mL LPS stimulation.The mRNA and protein expressions of NLRP3(P=0.009,P＜0.01),Caspase-1(P=0.018 1,P＜0.05),IL-1β(P=0.016,P＜0.01) in HSC3 were significantly increased after 12 h of 10 μg/mL LPS stimulation.The proliferation and migration of SCC25(P＜0.000 1,P＜0.000 1) and HSC3(P＜0.000 1,P＜0.000 1) were significantly increased after LPS stimulation.Conclusion：The expression of NLRP3/Caspase-1 inflammatory signaling pathway in OSCC cancer tissues was significantly higher than that in adjacent normal tissues,suggesting that NLRP3/Caspase-1 may play an important role in the occurrence and development of OSCC.LPS can further promote the proliferation and migration of oral cancer by activating NLRP3/Caspase-1 inflammatory signaling pathway,suggesting that oral pathogens can secrete LPS to promote the occurrence and development of OSCC.

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Memo

Memo:
国家自然（社会）科学基金预研项目(编号：CBY19-YZ08）;川北医学院附属医院第一批临床研究课题项目(编号：2021LC010）；川北医学院附属医院院级科研课题项目（编号：2019ZX003）
Last Update: 2023-03-30