|Table of Contents|

Effect of miR-194-3p on proliferation and apoptosis of gastric cancer cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 07
Page:
1206-1211
Research Field:
Publishing date:

Info

Title:
Effect of miR-194-3p on proliferation and apoptosis of gastric cancer cells and its mechanism
Author(s):
CUI Manli1; LU Ning1; ZHU Lin1; WANG Jia1; YAN Honglin1; ZHANG Mingxin1; 2
1.Department of Gastroenterology,the First Affiliated Hospital of Xi'an Medical University,Shaanxi Xi'an 710077,China；2.The Second Clinical Medical College of Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China.
Keywords:
miR-194-3p; gastric cancer; proliferation; apoptosis
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.07.005
Abstract:
Objective:To analyze the effect of miR-194-3p on the proliferation and apoptosis of gastric cancer cells and explore its mechanism.Methods:miR-194-3p expression and prognostic information from TCGA(The Cancer Genome Atlas) database and GEO(gene expression omnibus) database were extracted for statistical analysis.Real-time fluorescent quantitative PCR(RT-qPCR) was used to detect the expression of miR-194-3p in gastric cancer cell lines.CCK-8 experiment,clone formation experiment and flow cytometry were used to detect the effect of miR-194-3p on the proliferation and apoptosis of gastric cancer cell lines.Western blot was used to detect the expression of Caspase3 in HGC-27 and SGC-7901 cells after miR-194-3p transfection.Results:Compared with adjacent tissues,miR-194-3p was significantly higher expressed in gastric cancer tissues.In addition,prognostic analysis of TCGA showed that high expression of miR-194-3p was associated with a good prognosis.Compared with GES-1,miR-194-3p was significantly lower expressed in 6 kinds of gastric cancer cell lines.The results of CCK-8 and clone formation experiments showed that compared with the empty group,the lentivirus transfected and overexpressed miR-194-3p significantly inhibited the proliferation of gastric cancer cells.Flow cytometry showed that miR-194-3p can significantly promote the apoptosis of gastric cancer cells.Western blot results showed that the expression of Caspase3 was significantly increased in gastric cancer cells overexpressing miR-194-3p.Conclusion:miR-194-3p is low expressed in gastric cancer cells.Overexpression of miR-194-3p can significantly inhibit the proliferation of cancer cells and promote cell apoptosis.The mechanism of miR-194-3p promoting apoptosis may be related to the role of Caspase3 apoptosis related pathway.

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Memo

Memo:
陕西省重点研发计划（编号：2021SF-129，2022JM-502）；陕西省教育厅专项项目（编号：19JK0765）；陕西省西安市科技局计划项目［编号：2019114713YX002SF035(1)，2019114713YX002SF035(6)］
Last Update: 2023-02-28