|Table of Contents|

Influence of different cytological specimen preservation methods for thyroid fine needle puncture on BRAF V600E mutation detection

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 05
Page:
835-838
Research Field:
Publishing date:

Info

Title:
Influence of different cytological specimen preservation methods for thyroid fine needle puncture on BRAF V600E mutation detection
Author(s):
LUO Dan1; HAN Yanbo2; LI Shuang1; WANG Xiaofei1; TANG Hui1; LIU Fang1; SONG Xudong1; DONG Liru1
1.Pathology Department,North China Univercity of Science and Technology Affiliated Hospital,Hebei Tangshan 063000,China;2.Department of Orthopaedics,People's Hospital of Luanzhou City,Hebei Tangshan 063700,China.
Keywords:
papillary thyroid; BRAF V600E; fine needle aspiration cytology; preservation solution; extraction; scraping slice
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2023.05.009
Abstract:
Objective：To investigate the effect of different methods of preservation of detection of BRAF V600E mutation in fine needle aspiration cytology（FNAC） of thyroid.Methods：Retrospective analyzed study of 164 patients from Jan 2019 to Feb 2022 who underwent thyroid fine needle aspiration through ultrasound-guided in our hospital.DNA extracted respectively from materials preserved in commercial cell preservation solution,normal saline preservation solution and stained smears is used for the detection of BRAF V600E mutation.Results：There are no significant differences in the mean DNA concentration and the Ct value of reference gene among the three methods(P>0.05).For the A260/280 value,there was no statistically significant difference between the cell preservation solution group and the saline preservation solution group,nor between the saline preservation solution group and the scraping slice group (P>0.05),while there was statistically significant difference in the A260/280 value between the cell preservation solution group and the scraping slice group(P<0.05).Conclusion：Scraping thyroid FNAC samples can obtain high DNA purity and low laboratory requirements,which is suitable for promotion in primary hospitals.

References:

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Memo

Memo:
河北省省属高等学校科技基础研究项目（自然科学）（编号:JQN2020015）；河北省重点科技研究计划课题（编号:20210618）；河北省适宜卫生技术项目（编号:2018013）；河北省医学适用技术跟踪项目（编号:G2019068）
Last Update: 2023-01-31