|Table of Contents|

The effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 05
Page:
800-805
Research Field:
Publishing date:

Info

Title:
The effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line
Author(s):
LI Jun1; BU Yafei2; ZHOU Haining1; WANG Lei1
1.Gastrointestinal Surgery Department;2.Emergency Department,General Hospital of Ningxia Medical University,Ningxia Yinchuan 750004,China.
Keywords:
gastric cancer; LINC01060; epithelial-mesenchymal transition
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.05.003
Abstract:
Objective：To explore the effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line.Methods:The expression of LINC01060 in gastric cancer was analyzed by GEO database,and the stable BGC-823 cell line with overexpression and silencing of LINC01060 was established.The expression of LINC01060 was verified by Real time PCR.Cell proliferation was detected by CCK-8 assay.Cell apoptosis was detected by flow cytometry.Cell metastasis was detected by scratch assay.Cell invasion was detected by Transwell assay,and the expressions of EMT-related proteins E-cadherin,Vimentin,N-cadherin and Snail were detected by Western blot.Results:The GEO database found that the expression of LINC01060 was low in gastric cancer (P＜0.05).Real time PCR results showed that the expression of LINC01060 in the silence group was significantly down-regulated (P＜0.05),and the expression of LINC01060 in the over-exposure group was significantly up-regulated (P＜0.05).CCK-8 showed that the proliferation ability of cells in the overexpression group was significantly decreased (P＜0.05).Flow cytometry showed that the proportion of cells apoptosis in the overexpression group was significantly increased (P＜0.05).Transwell results showed that the cell invasion ability of the overexpression group was significantly decreased (P＜0.05).Cell scratch showed that the cell migration ability of the overexpression group was significantly decreased (P＜0.05).Western blot showed that the expression level of E-cadherin protein in the overexpression group was significantly up-regulated (P＜0.05),and Vimentin,N-cadherin,Snail protein expression level was significantly down-regulated (P＜0.05).The above experiments were the opposite in silence group.Conclusion:LINC01060 can promote apoptosis and inhibit proliferation,invasion,migration and EMT in human gastric cancer BGC-823 cell line.

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Memo

Memo:
宁夏自然科学基金项目（编号：2021AAC03392）
Last Update: 2023-01-31