|Table of Contents|

Study on the effect of ultrasound combined with gambogic acid nanoparticles on human glioma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 05
Page:
789-794
Research Field:
Publishing date:

Info

Title:
Study on the effect of ultrasound combined with gambogic acid nanoparticles on human glioma
Author(s):
DONG Lei; WEI Xixi; LI Menghao; HE Mei; LI Cheng; LI Zhaoyi; YIN Chaohua; ZHANG Suting; LI Nana; WANG Yongling; CHANG Liansheng; WANG Feng
Henan Key Laboratory of Medical Tissue Regeneration,School of Basic Medical Sciences,Xinxiang Medical University,Henan Xinxiang 453003,China.
Keywords:
human glioma; microbubbles; gambogic acid; sonopore effect
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2023.05.001
Abstract:
Objective: To study the effective concentration of luteinic acid on human glioma cell line U87 and the killing effect of porous lipid/PLGA hybrid microbubbles (GA/PLGA) loaded with gambogic acid,GA on U87 under ultrasound.Methods:The particle size potential distribution of GA/PLGA was detected by Malvern particle size analyzer,the cell viability was detected by CCK-8,and the apoptosis rate was detected by Annexin V/PI double staining.Results:The GA/PLGA prepared by adding 4 mg GA every 50 mg PLGA had the highest entrapment efficiency and drug loading,which were (95.57±1.89)% and (7.65±0.15)%,respectively.The 50% inhibitory concentration (IC50) of U87 cells treated with 1.5 μmol/L.The lowest viability of U87 cells in US+GA/PLGA group was (3.85±0.67)%.The apoptosis rate of US+GA/PLGA group was the highest,which was (75.97±0.49)%.Conclusion:GA/PLGA microbubbles can enhance the killing effect of GA on U87 under ultrasound.

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Memo

Memo:
国家自然科学基金河南省联合基金（编号：U1804187）
Last Update: 2023-01-31