|Table of Contents|

Lycorine inhibits the proliferation of esophageal cancer cells through regulating G3BP1/TGF-β/Smad signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 04
Page:
603-609
Research Field:
Publishing date:

Info

Title:
Lycorine inhibits the proliferation of esophageal cancer cells through regulating G3BP1/TGF-β/Smad signaling pathway
Author(s):
REN Liping1; 2 ; JIN Shaoju1; 2 ; MA Yongchao2 ; XU Songtao2; 3
1.Department of Pharmacy;3.Medical Department,Luohe Medical College,Henan Luohe 462002,China;2.Henan Province Engineering ＆ Technology Research Center of Foods for Special Medical Purpose,Henan Luohe 462002,China.
Keywords:
lycorine; G3BP1/TGF-β/Smad signaling pathway; esophageal cancer; cell proliferation
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2023.04.003
Abstract:
Objective:To explore the effect of lycorine on the proliferation of esophageal cancer cells and analyze its mechanism.Methods:The inhibition of cells was analyzed by cell counting kit-8 (CCK-8) method,and the levels of GTPase activating protein （SH3 domain) binding protein 1 (G3BP1),transforming growth factor-β1 (TGF-β1),p-Smad2/Smad2 and p-Smad3/Smad3 were analyzed by Western blot.After G3BP1 knockdown or overexpression,the inhibition rate of cell proliferation and the levels of G3BP1,TGF-β1,p-Smad2/Smad2 were observed.The tumorigenesis experiment in nude mice verified the effects of lycorine on the tumor weight,tumor volume and the levels of G3BP1,TGF-β1,p-Smad2/Smad2 in nude mice.Results:2~64 μmol/L lycorine inhibited the proliferation of ECA109 cells,and the IC50 of lycorine was (16.21±2.35) μmol/L for 48 hours.4,8,16 μmol/L lycorine could down-regulate the level of G3BP1,TGF-β1,p-Smad2/Smad2,p-Smad3/Smad3.Knocking down the expression of G3BP1 could obviously improve the inhibitory effect of lycorine on the proliferation of ECA109 cells,and promote lycorine to reduce the levels of G3BP1,TGF-β1 and p-Smad2/Smad2 (P＜0.05).Up-regulating the expression of G3BP could obviously down-regulate the inhibitory effect of lycorine on the proliferation of ECA109 cells,and inhibit lycorine to decrease the levels of G3BP1,TGF-β1 and p-Smad2/Smad2 (P＜0.05).In vivo experiments showed compared with the model control group,25,50 and 100 mg/kg lycorine could reduce the tumor volume and mass,and down-regulate the levels of G3BP1,TGF-β1 and p-Smad2/Smad2 (P＜0.05).Conclusion:Lycorine can inhibit the proliferation of ECA109 cells,and has obvious antitumor effect on transplanted ECA109 cells of nude mice in vivo.Its mechanism is related to the regulation of G3BP1/TGF-β/Smad signaling pathway.

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Memo

Memo:
河南省高等学校重点科研项目（编号：22B310006）；漯河医学高等专科学校创新创业发展能力提升工程项目（编号：2021LYZKJXM024，2020-LYZKYYB005）
Last Update: 1900-01-01