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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 03

Page:

528-534

Research Field:

Publishing date:

Info

Title:

Expression of TGF-β1/Rac1pathway protein in prostate cancer tissue and its relationship between the biochemical recurrence after radical operation

Author(s):

LIN Yunqiao¹; LI Zhiqiang¹; WENG Wubin²; CHEN Congqi¹; ZHANG Yun¹

1.Ningde Municipal Hospital of Ningde Normal University,Fujian Ningde 352100,China；2.Mindong Hospital of Ningde City,Fujian Ningde 352100,China.

Keywords:

prostate cancer; radical operation; transforming growth factor-β1; ras related C3 botulinum toxin substrate 1; Vimentin; E-cadherin; biochemical recurrence

PACS:

R737.25

DOI:

10.3969/j.issn.1672-4992.2023.03.027

Abstract:

Objective:To investigate the expression of transforming growth factor-β1（TGF-β1)/Ras related C3 botulinum toxin substrate 1(Rac1) pathway protein in prostate cancer tissue its relationship between the biochemical recurrence after radical operation.Methods:The clinical data of 200 patients who underwent radical prostatectomy for prostate cancer in our hospital from February 2015 to February 2018 were retrospectively analyzed,and the specimens of prostate cancer tissues and paracancerous tissues were preserved during operation.The expression of TGF-β1,Rac1,Vimentin and E-cadherin proteins in prostate cancer tissues and paracancerous tissues were detected by immunohistochemical method and were compared.In addition,the biochemical recurrence of the patients during the 3-year follow-up period after radical operation was analyzed and classified into biochemical recurrence group and non biochemical recurrence group,and the positive expression rate of TGF-β1,Rac1,Vimentin and E-cadherin proteins in prostate cancer tissues of biochemical recurrence group and non biochemical recurrence group were compared,and Cox regression model was used to analyze the relationships between expressions of TGF-β1,Rac1,Vimentin and E-cadherin proteins in prostate cancer tissues and biochemical recurrence after radical operation.Results:The positive expression rates of TGF-β1,Rac1,Vimentin proteinsin prostate cancer tissues were significantly higher than those of the paracancerous tissues,but the positive expression rate of E-cadherin was significantly lower than that of the adjacent tissues,and the differences were statistically significant(P＜0.05).After 3 years follow-up,the biochemical recurrence rate was 14.50%(29/200),and the positive expression rates of TGF-β1,Rac1,Vimentin proteins in biochemical recurrence group were higher than those in non biochemical recurrence group(P＜0.05),but the positive expression rate of E-cadherin protein was lower than that of non biochemical recurrence group(P＜0.05).Cox regression model analysis showed that preoperative PSA level,Gleason score of specimens,capsular invasion,seminal vesicle invasion,perineural invasion,vascular invasion,surgical margin positive,positive expression of TGF-β1 protein,positive expression of Rac1 protein and positive expression of Vimentin protein were positive were the risk factors of biochemical recurrence after radical prostatectomy(HR=3.350,3.117,4.702,5.618,6.328,5.114,6.007,3.019,3.077,2.740,P＜0.05),but the positive expression of E-cadherin was the protective factor(HR=0.471,P＜0.05).Conclusion:The TGF-β1,Rac1 and Vimentin proteins in prostate cancer tissues are highly expressed,while E-cadherin protein is low expressed,which have a certain relationship between the above proteins expression and biochemical recurrence after radical operation,and the positive expression of TGF-β1,Rac1 and Vimentin proteins can increase the probability of biochemical recurrence,but the positive expression of E-cadherin protein can reduce the risk of postoperative recurrence,and it has a certain guiding role in clinical practice.

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Last Update: 2022-12-30