|Table of Contents|

Plumbagin inhibits proliferation and promotes apoptosis of colon cancer cells through CXCL8/PI3K/AKT glycolysis pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 03
Page:
411-416
Research Field:
Publishing date:

Info

Title:
Plumbagin inhibits proliferation and promotes apoptosis of colon cancer cells through CXCL8/PI3K/AKT glycolysis pathway
Author(s):
YANG Fang1; HOU Qianqian1; LI Na1; WANG Daqing1; CHEN Xiaohua2
1.Hengshui People's Hospital,Hebei Hengshui 053000,China;2.The Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China.
Keywords:
plumbagin; colon cancer; glycolysis; CXCL8; PI3K/AKT signal pathway
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.03.004
Abstract:
Objective:To observe the effect of plumbagin on cell proliferation and apoptosis of Caco-2,and explore its potential mechanism.Methods:Cell count（CCK8) and flow cytometry were used to measure the proliferation inhibition rate and apoptosis rate of Caco-2 cells treated with plumbagin（4,8,12 μmol/L).Caco-2 cells without any treatment were set as the Control group.si-NC group（transfected si-NC) and si-C-X-C motif chemokine ligand 8（CXCL8) group（transfected si-CXCL8) were transfected into Caco-2 cells by liposome method.Caco-2 cells treated with 8 μmol/L plumbagin and 0.5% DMSO were set as 8 μmol/L+DMSO group.Caco-2 cells treated with 8 μmol/L plumbagin and z-VAD-FMK or 740Y-P were set as 8 μmol/L+z-VAD-FMK group,8 μmol/L+740Y-P group,respectivly.The mRNA and protein expression of CXCL8 were measred by real-time fluorescence quantitative polymerase chain reaction（RT-qPCR) and Western blot（WB).CXCL8,M2 pyruvate kinase（PKM2),glyceraldehyde-3-phosphate dehydrogenase（LDHA),human α-enolase（ENO1),glucose phosphate isomerase（GPI),phosphorylated phosphatidylinositol 3 kinase（p-PI3K) and phosphorylated protein kinase B（p-Akt) were measred by Western blot.Results:Compared with the Control group,plumbagin（4,8,12 μmol/L) promoted the proliferation inhibition rate and apoptosis rate of Caco-2 cells and inhibited the mRNA and protein expression of CXCL8 in a concentration dependent manner.Compared with 8 μmol/L+DMSO group,the proliferation inhibition rate and apoptosis rate significantly decreased,and the mRNA and protein expression of CXCL8 significantly increased in 8 μmol/L+z-VAD-FMK group（P<0.05).Plumbagin（4,8,12 μmol/L) inhibited the protein expression of PKM2,LDHA,p-PI3K and p-AKT in a concentration dependent manner.The protein expressions of PKM2,LDHA,p-PI3K and p-AKT in si-CXCL8 group were significantly lower than those in si-NC group.740Y-P significantly weakened the promoting effect of plumbagin on the proliferation inhibition rate and apoptosis rate of Caco-2 cells.Conclusion:Plumbagin could inhibit the proliferation and promote apoptosis of colon cancer cells,and its potential mechanism may be related to CXCL8/PI3K/AKT glycolysis pathway.

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Last Update: 2022-12-30