|Table of Contents|

miR-138-5p inhibits proliferation,migration and invasion of glioma cells via targeting RAB22A

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 03
Page:
401-406
Research Field:
Publishing date:

Info

Title:
miR-138-5p inhibits proliferation,migration and invasion of glioma cells via targeting RAB22A
Author(s):
LI Xu; WANG Puxiu; LIU Guoli
Department of Pharmacy,the First Hospital of China Medical University,Liaoning Shenyang 110001,China.
Keywords:
miR-138-5p; RAB22A; glioma; proliferation; migration; invasion
PACS:
R739.41
DOI:
10.3969/j.issn.1672-4992.2023.03.002
Abstract:
Objective:To investigate the effect of miR-138-5p on the proliferation,migration and invading of glioma cells and its mechanism.Methods:U251 cells were divided into miR-NC（control) group,miR-mimics group,miR-inhibitor group and miR-mimics+RAB22A-pcDNA3.1 group.The expression of miR-138-5p in normal human astrocytes and glioma cells was detected by real-time fluorescence quantitative PCR.CCK8 assay was used to explore the proliferation of U251 cells.Transwell assay was used to explore the migration and invasion ability of U251 cells.Targetscan database predicted the downstream target genes of miR-138-5p.Double luciferase reporter gene assay was used to detect that RAB22A was the target gene of miR-138-5p.The interaction between miR-138-5p and RAB22A was further verified by RNA binding protein immunoprecipitation assay.Western blot assay was used to detect RAB22A protein expression in tissues and cells.Results:Compared with normal astrocytes,the expression of miR-138-5p was decreased in glioma cells（P＜0.05).CCK8 and Transwell assay results showed that overexpression of miR-138-5p significantly inhibited the proliferation,migration and invasion of U251 cells（P＜0.05).Targetscan database was used to predict that the downstream target gene of miR-138-5p was RAB22A,and miR-138-5p acted on the 3' UTR region of RAB22A.The interaction between miR-138-5p and RAB22A was verified by dual luciferase reporter gene assay and RNA binding protein immunoprecipitation assay.RAB22A was significantly overexpressed in glioma cells（P＜0.05),and overexpression of miR-138-5p significantly inhibited RAB22A expression in U251 cells（P＜0.05).Conclusion:The expression of miR-138-5p is decreased in glioma cells,and miR-138-5p restrains cell proliferation,migration and invasion by down-regulating RAB22A expression.

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Memo

Memo:
National Natural Science Foundation of China（No.81803440）;国家自然科学基金资助项目（编号：81803440)
Last Update: 2022-12-30