|Table of Contents|

Mechanism of lncRNA RP11-297P16.4 promoting invasion and migration of lung adenocarcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 03
Page:
395-401
Research Field:
Publishing date:

Info

Title:
Mechanism of lncRNA RP11-297P16.4 promoting invasion and migration of lung adenocarcinoma cells
Author(s):
XU Baixue1; WANG Wei1; PANG Min2; FAN Shasha1; WANG Zhixin1; ZHANG Lizhong1; AN Caixing1; ZHANG Peilin1; CHENG Baolong1; LIU Fangfang1; WANG Hailong1; YU Baofeng1
1.Department of Biochemistry and Molecular Biology,School of Basic Medicine,Basic Medical Sciences Center,Shanxi Medical University,Shanxi Jinzhong 030600,China;2.Department of Pulmonary and Critical Care Medcine,the First Hospital,Shanxi Medical University,Shanxi Province Key Laboratory of Respiratory Disease,Shanxi Taiyuan 030001,China.
Keywords:
lung adenocarcinoma; lncRNA RP11-297P16.4; invasion; migration; matrix metalloproteinase-2; matrix metalloproteinase-9
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.03.001
Abstract:
Objective:To investigate the effect of long non-coding RNA（lncRNA) RP11-297P16.4 on the invasion and migration of human lung adenocarcinoma cells of H1299 and A549.Methods:The relative expression levels of RP11-297P16.4 in H1299,A549 and human normal bronchial epithelial cells HBEC were detected by real-time fluorescence quantitative PCR.Special shRNA for RP11-297P16.4 was designed and synthesized.H1299,A549 cells with stable knockdown of lncRNA RP11-297P16.4 were established by lentivirus infection,then they were divided into control/H1299 group,experimental/H1299 group,control/A549 group and experimental/A549 group.The control group was infected with shRNA-NC,and the experimental group was infected with shRNA-RP11-297P16.4.qRT-PCR was used to detect the shRNA inhibition efficiency.Cell scratch and Transwell assays were used to establish the effect of RP11-297P16.4 on the invasion and migration of lung adenocarcinoma cells.qRT-PCR and Western blot were performed to assay the mRNA and protein expression levels of matrix metalloproteinase（MMP)-2 and-9.Results:Compared with the control group,RP11-297P16.4 was highly expressed in lung adenocarcinoma cells,and the difference was statistically significant（P＜0.001,P＜0.01).qRT-PCR analysis determined that the mRNA expression level of RP11-297P16.4 was significantly reduced after shRNA knockdown(P＜0.001,P＜0.001).The scratch assay demonstrated that the wound healing rate of cells in the experiment group at 24 h and 48 h was lower than that in the control group,and the difference was statistically significant（P＜0.05,P＜0.01,P＜0.001,P＜0.001).Transwell experiment showed that the number of cellular invasion and migration in the experiment group was significantly less than that in the control group,and the difference was statistically significant（P＜0.001,P＜0.001).The results of qRT-PCR and Western blot revealed that the mRNA and protein expression levels of MMP-2 and MMP-9 in the experiment group were lower than those in the control group,and the difference was statistically significant(all P＜0.05).Conclusion:The lncRNA RP11-297P16.4 enhanced the invasive and migratory abilities of H1299 and A549 cells by promoting MMP-2 and MMP-9 expression.

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