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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 02

Page:

203-210

Research Field:

Publishing date:

Info

Title:

Effects of MTHFD1L on proliferation,apoptosis and tumorigenesis of tongue squamous cell carcinoma cells and its mechanism

Author(s):

XU Zhiming¹; BAI Yuting²; ZHOU Xiaokai³; CHEN Yuxin³; MENG Jian¹; 4

1.Xuzhou Clinical College of Xuzhou Medical University,Jiangsu Xuzhou 221000,China;2.School of Stomatology,Wuhan University,Hubei Wuhan 430000,China;3.School of Stomatology,Xuzhou Medical University,Jiangsu Xuzhou 221000,China;4.Xuzhou Central Hospital,Jiangsu Xuzhou 221000,China.

Keywords:

MTHFD1L; tongue squamous cell carcinoma; proliferation; apoptosis; p38α; IL1α

PACS:

R739.86

DOI:

10.3969/j.issn.1672-4992.2023.02.002

Abstract:

Objective:To investigate the effect of methylenetetrahydrofolate dehydrogenase 1 (MTHFD1L) knockdown on the proliferation,clone formation,apoptosis and tumorigenesis of Cal27 and its mechanism.Methods:Data related to head and neck squamous cell carcinoma (HNSC) was downloaded from TCGA and GTEx databases to compare the difference of MTHFD1L expression and survival analysis between HNSC and normal tissues.GEPIA database was used to verify differential expression and predict survival curves.The expression of MTHFD1L was knocked down by lentivirus small interfering RNA technology,and the knockdown efficiency of MTHFD1L was detected by RT-qPCR and Western blot analysis.The effects of MTHFD1L knockdown on Cal27 cell proliferation,clonal formation and apoptosis were investigated by Celigo cell count assay,MTT assay,plate cloning assay and Annexin V-APC staining,respectively.The nude mouse tongue cancer model was established by injecting Cal27 cells into the armpit.The weight,volume and fluorescence intensity were compared.The expression levels of p38α and IL1α were detected by RT-qPCR and Western blot.Results:The expression of MTHFD1L in HNSC was higher than that in normal tissues,and the high expression of MTHFD1L was associated with poor prognosis.MTHFD1L knockdown rate was 95.1%.The results of Celigo cell counting,MTT and plate cloning showed that MTHFD1L knockdown inhibited the proliferation rate and clonal formation ability of Cal27.Annexin V-APC staining showed that MTHFD1L knockdown significantly promoted apoptosis of Cal27.The tumor volume,weight,fluorescence intensity and tumor formation rate of nude mice inoculated with MTHFD1L knockdown Cal27 cells decreased significantly.After MTHFD1L knockdown,the expression of p38α and mRNA in Cal27 cells decreased significantly,while the expression of IL1α increased significantly.Conclusion:MTHFD1L knockdown inhibited tongue squamous cell carcinoma cell proliferation,induced apoptosis,and inhibited tumor formation in mice.

References:

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Memo

Memo:

江苏省卫生计生委科研资助项目（编号:H2017080）；江苏省徐州市科技计划项目（编号：KC21187）

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