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The effect of bevacizumab on the angiogenesis mimicry of human choroidal melanoma MUM-2B cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 02
Page:
197-202
Research Field:
Publishing date:

Info

Title:
The effect of bevacizumab on the angiogenesis mimicry of human choroidal melanoma MUM-2B cells
Author(s):
ZHOU Yujuan1; DING Ruilin2; PENG Hongju3; LI Yuan4; NI Laichao5; WEN Qinglian6
Keywords:
bevacizumab; human choroidal melanoma; angiogenic mimicry; proliferation; invasion; HIF-1a/VE-cadherin/EphA2/PI3K-Akt
PACS:
R739.5
DOI:
10.3969/j.issn.1672-4992.2023.02.001
Abstract:
Objective:To investigate the effect of bevacizumab on the angiogenic mimicry of human choroidal melanoma cell (MUM-2B) and the possible related molecular pathways in vitro and in vivo.Methods:MUM-2B cells were treated with different concentrations of bevacizumab (0,0.1,1,2,3 mg/mL) to observe its effect on the tube-forming ability of MUM-2B cells.The effects of the proliferation and invasion of MUM-2B cells were detected by CCK-8 and Transwell assay.Then,the subcutaneous transplantation tumor models were established in nude mice,and the effect of bevacizumab on VM formation of subcutaneous transplantation tumors in it was investigated by different doses of bevacizumab (5 mg/kg,7.5 mg/kg,10 mg/kg) intervention,and the expression of VM was detected by double staining with CD31/PAS immunohistochemistry.The changes in the expression levels of HIF-1a,VE-cadherin,EphA2,and PI3K proteins were detected by immunohistochemistry.Results:In vitro,different concentrations of bevacizumab (0,0.1,1,2,3 mg/mL) treated with MUM-2B cells for 24 hours did not show significant inhibitory or promotional effects on the tube-forming ability,proliferation,and invasion ability of MUM-2B cells.The difference between the experimental group and the control group was not statistically significant (P＞0.05).In vivo,compared with the control group,the bevacizumab experimental group (5 mg/kg,7.5 mg/kg,10 mg/kg) could significantly promote the ability of MUM-2B cells to form VM (P＜0.05),and the number of tubes was positively correlated with the concentration of bevacizumab (r=0.942,P＜0.05).The expression levels of HIF-1a,VE-cadherin,EphA2,and PI3K-Akt proteins were significantly higher in the experimental group than in the control group (P＜0.05),and the expression of each molecule increased in a concentration-dependently manner with the increase of bevacizumab concentration.Conclusion:In vitro,bevacizumab did not significantly inhibit or promote the tubulation,proliferation and invasion of MUM-2B cells.In vivo,Bevacizumab can promote the expression of HIF-1a and up-regulate the subsequent cascade signal pathway of VE-cadherin/EphA2/PI3K-Akt to accelerate the generation of VM.

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北京医卫健康公益基金会（编号:HR20002）
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