|Table of Contents|

Effect of down-regulation of RAGE on proliferation,apoptotic capacity and cycle distribution of bladder cancer T24 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 06
Page:
1001-1006
Research Field:
Publishing date:

Info

Title:
Effect of down-regulation of RAGE on proliferation,apoptotic capacity and cycle distribution of bladder cancer T24 cells
Author(s):
PENG Weixing; HE Jingbing
Department of Urology,Shanghai Ruijin Hospital Zhoushan Branch Urology,Zhejiang Zhoushan 316100,China.
Keywords:
bladder cancer; cell apoptosis; cycle distribution; cell proliferation
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2022.06.010
Abstract:
Objective:To investigate the effect of downregulation of receptor for advanced glycation end products (RAGE) on the proliferation,apoptotic capacity and cycle distribution of bladder cancer T24 cells.Methods:After the human bladder cancer T24 cell line was cultured,the down-regulated RAGE group and the up-regulated RAGE group were constructed by lentiviral vector.Cell proliferation ability was detected by MTT,cell apoptosis and cycle distribution were detected by flow cytometry,cell invasion ability was detected by Transwell chamber test,cell migration ability was detected by cell scratch test,and relative expression of CDK4,p-Akt,ICAM-1,MMP-9,Bcl-2,Bak and caspase7 was detected by Western blot.Results:At 24,48 and 72 h,the cell proliferation rate in the up-regulated RAGE group was higher than that in the control group and the down-regulated RAGE group,and the apoptosis rate was lower than that in the control group and the down-regulated RAGE group (P＜0.05).The proportion of cells in the up-regulated RAGE group in the G0/G1 phase was lower than that in the control group and the down-regulated RAGE group (P＜0.05).The relative expression of CDK4,p-Akt,ICAM-1,MMP-9,Bcl-2,caspase7 protein and the number of invasive cells and migrating cells in RAGE group were lower than those in control group,and the relative expression of Bak protein was higher than that in control group.The relative expression of CDK4,p-Akt,ICAM-1,MMP-9,Bcl-2,caspase7 protein and the number of invasive cells and migrating cells in RAGE group were higher than those in control group,and Bak protein was lower than that in RAGE group.The relative protein expression was lower than that in the control group (P＜0.05).Conclusion:Downregulation of RAGE expression can inhibit the proliferation of bladder cancer T24 cells,regulate the relative expression of p-Akt,CDK4,ICAM-1,MMP-9,block the cell cycle distribution,inhibit cell invasion and migration,regulate the relative expression of caspase7,Bcl-2,Bak,and promote the apoptosis of bladder cancer T24 cells.

References:

[1]CRISPEN PL,KUSMARTSEV S.Mechanisms of immune evasion in bladder cancer［J］.Cancer Immunol Immunother,2020,69(1):3-14.
[2]LIN F,YIN HB,LI XY,et al.Bladder cancer cell secretedexosomal miR-21 activates the PI3K/AKT pathway in macrophages to promote cancerprogression［J］.Int J Oncol,2020,56(1):151-164.
[3]AMOUROUX M,SALLERON J,HUIN-SCHOHN C,et al.Clinical evaluation of a device providing simultaneous white-light and fluorescence video streams as well as panoramic imaging during fluorescence assisted-transurethral resection of bladder cancer［J］.J Opt Soc Am A Opt Image Sci Vis,2019,36(11):C62-C68.
[4]杨勇,姜睿.膀胱癌YAP表达及YAP对膀胱癌干细胞特性的影响［J］.实用医学杂志,2018,34(19):3181-3184. YANG Y,JIANG R.YAP expression in bladder cancer and the effect of YAP on the characteristics of bladder cancer stem cells ［J］.Journal of Practical Medicine,2018,34 (19):3181-3184.
[5]SU Y,DU Z,ZHONG G,et al.circ5912suppresses cancer progression via inducing MET in bladder cancer［J］.Aging (AlbanyNY),2019,11(23):10826-10838.
[6]OHADIAN MOGHADAM S,NOWROOZI MR.Toll-like receptors:The role in bladdercancer development,progression and immunotherapy［J］.Scand J Immunol,2019,90(6):e12818.
[7]王亦然,王羽含,王志平,等.膀胱癌靶向治疗研究进展［J］.中华泌尿外科杂志,2018,39(8):632-634. WANG YR,WANG YH,WANG ZP,et al.Research progress of targeted therapy for bladder cancer ［J］.Chinese Journal of Urology,2018,39 (8):632-634.
[8]SEIDL C.Targets for therapy of bladder cancer［J］.Semin Nucl Med,2020,50(2):162-170.
[9]李洋,于奇.RAGE、P-P38在非小细胞肺癌中的表达及相关性研究［J］.临床肺科杂志,2016,21(5):862-865. LI Y,YU Q.Expression and correlation study of RAGE and P-P38 in non-small cell lung cancer ［J］.Journal of Clinical Pulmonary Medicine,2016,21(5):862-865.
[10]刘莹,蒋敦科,李素艳,等.2型糖尿病合并结肠癌老年患者肿瘤组织中晚期糖基化终产物受体及转化生长因子-β1的表达及临床意义［J］.中国老年学杂志,2019,39(6):1334-1337. LIU Y,JIANG DK,LI SY,et al.Expression and clinical significance of advanced glycation end product receptor and transforming growth factor-beta 1 in tumor tissues of elderly patients with type 2 diabetes mellitus and colon cancer ［J］.Chinese Journal of Gerontology,2019,39(6):1334-1337.
[11]刘清,丁光仁,惠永峰,等.HMGB1及其受体RAGE分子信号通路参与调节肝细胞肝癌增殖和侵袭迁移机制的研究［J］.临床和实验医学杂志,2017,16(3):213-217. LIU Q,DING GR,HUI YF,et al.HMGB1 and its receptor RAGE molecular signaling pathway are involved in regulating the proliferation and invasion and migration mechanism of hepatocellular carcinoma ［J］.Journal of Clinical and Experimental Medicine,2017,16(3):213-217.
[12]段堃,李岩岩,赵艳,等.CUG连接蛋白1在膀胱癌中的表达及其对BIU-87细胞株增殖及侵袭能力的影响［J］.实用医学杂志,2016,32(3):411-414. DUAN K,LI YY,ZHAO Y,et al.Expression of CUG connexin 1 in bladder cancer and its effect on proliferation and invasion ability of BIU-87 cell line ［J］.Journal of Practical Medicine,2016,32(3):411-414.
[13]曹峻,张幸,梁梅花,等.芒柄花黄素对膀胱癌细胞凋亡作用及对β-catenin通路的影响［J］.广东医学,2016,37(21):3189-3192. CAO J,ZHANG X,LIANG MH,et al.Effects of erythroxanthin on bladder cancer cell apoptosis and on the beta-catenin pathway ［J］.Guangdong Medicine,2016,37 (21):3189-3192.
[14]郭俊生,夏海波,门超,等.微小RNA-20a对膀胱癌T24细胞增殖和凋亡的影响［J］.临床肿瘤学杂志,2018,23(2):116-121. GUO JS,XIA HB,MEN C,et al.Effects of microRNA-20a on proliferation and apoptosis of bladder cancer T24 cells ［J］.Journal of Clinical Oncology,2018,23(2):116-121.
[15]CHEN S,ZHOU Q,GUO Z,et al.Inhibition of MELK produces potential anti-tumour effects in bladder cancer byinducing G1/S cell cycle arrest via the ATM/CHK2/p53 pathway［J］.J Cell Mol Med,2020,24(2):1804-1821.
[16]谢秋娴,郑曼佳,谢昭雄,等.膀胱癌相关蛋白对宫颈癌细胞的影响及其在细胞周期信号传导网络中的作用［J］.广东医学,2016,37(1):62-66. XIE QX,ZHENG MJ,XIE ZX,et al.Effects of bladder cancer-related proteins on cervical cancer cells and their roles in cell cycle signaling network［J］.Guangdong Medicine,2016,37(1):62-66.
[17]许振,刘志强,孟玮,等.宫颈癌组织中maspin、AKT、p-AKT表达变化及其与肿瘤微淋巴管密度的相关性［J］.山东医药,2017,57(27):23-26. XU Z,LIU ZQ,MENG W,et al.Changes of maspin,AKT,p-AKT expression in cervical cancer tissues and their correlation with tumor microlymphatic vessel density ［J］.Shandong Medicine,2017,57(27):23-26.
[18]夏靖华,张志培,文苗苗,等.CREPT和CDK4在非小细胞肺癌组织中的表达及临床意义［J］.临床肿瘤学杂志,2018,23(4):340-345. XIA JH,ZHANG ZP,WEN MM,et al.Expression and clinical significance of CREPT and CDK4 in non-small cell lung cancer ［J］.Journal of Clinical Oncology,2018,23(4):340-345.
[19]谢斌,谢雄,朱卫丰,等.清燥救肺汤对结肠癌增殖侵袭相关蛋白c-myc、EGF、ICAM-1、MMP2、TGF-α表达的影响［J］.广州中医药大学学报,2017,34(1):86-90. XIE B,XIE X,ZHU WF,et al.Effects of Qingzao Jiufei Decoction on the expression of c-myc,EGF,ICAM-1,MMP2,TGF-a,which are related to proliferation and invasion of colon cancer ［J］.Journal of Guangzhou University of Traditional Chinese Medicine,2017,34(1):86-90.
[20]程宇,束军,徐胜,等.PEDF对肺腺癌A549细胞迁移、侵袭能力和转移相关蛋白表达水平的影响［J］.安徽医科大学学报,2016,51(3):345-350. CHENG Y,SHU J,XU S,et al.Effects of PEDF on migration,invasive ability and expression level of metastasis-related proteins in lung adenocarcinoma A549 cells ［J］.Journal of Anhui Medical University,2016,51(3):345-350.
[21]WANG F,CAO M,FAN M,et al.AMPK-mTOR-ULK1axis activation-dependent autophagy promotes hydroxycamptothecin-inducedapoptosis in human bladder cancer cells［J］.J Cell Physiol,2020,235(5):4302-4315.
[22]卢志兴,刘圣光,赵丁丁,等.马尾神经压迫损伤后细胞凋亡相关蛋白、氧化应激损伤相关蛋白的表达规律［J］.海南医学院学报,2017,23(10):1312-1314,1318. LU ZX,LIU SG,ZHAO DD,et al.Expression pattern of apoptosis-related proteins and oxidative stress-related proteins after compression injury of cauda equina ［J］.Journal of Hainan Medical College,2017,23(10):1312-1314,1318.

Memo

Memo:
-
Last Update: 1900-01-01