|Table of Contents|

Study on the mechanism and inhibition of proliferation of diffuse large B-cell lymphoma cell by methyltransferase inhibitors

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 06
Page:
966-971
Research Field:
Publishing date:

Info

Title:
Study on the mechanism and inhibition of proliferation of diffuse large B-cell lymphoma cell by methyltransferase inhibitors
Author(s):
GAO Haiyang1; 2; 3; 4; WANG Zhiqiang2; 3; ZANG Lanlan2; 3; WANG Lijuan1; 2; 3
1.Eleventh Clinical College,Qingdao University,Shandong Qingdao 266071,China;2.Central Laboratory,Linyi People's Hospital,Shandong Linyi 276100,China;3.Linyi Key Laboratory of Tumor Biology,Shandong Linyi 276100,China;4.Jining First People's Hospital,Shandong Jining 272000,China.
Keywords:
diffuse large B cell lymphoma; methyltransferase inhibitor; ferroptosis; apoptosis
PACS:
R733
DOI:
10.3969/j.issn.1672-4992.2022.06.004
Abstract:
Objective:To explore the effective cytotoxicity concentration of methyltransferase inhibitor DCG066 on human diffuse large B-cell lymphoma,and then to study its mechanism.Methods:The effective cytotoxicity concentration of DCG066 on human diffuse large B-cell lymphoma TMD-8 cells was explored by CCK-8 experiment,and the binding of Calcein-AM/PI reagent to TMD-8 live and dead cells after DCG066 treatment was detected by immunofluorescence experiment.Flow cytometry was used to detect the induction of TMD-8 cell apoptosis induced by DCG066,cell cycle inhibition,and intracellular reactive oxygen species levels.Western Blot and transcriptome sequencing were used to analyze the expression of apoptosis,cell cycle and ferroptosis-related genes.Results:Compared with the control group,DCG066 exhibits cytotoxicity to TMD-8 cells with a dose-dependent manner.DCG066 can induce apoptosis and cell cycle inhibition in TMD-8 cells.The expression of apoptosis-related proteins such as Bax and DDIT is significantly up-regulated,and the expression of cell cycle-related proteins such as Cyclin E1 and Cyclin A2 is down-regulated.In addition,after TMD-8 cells were treated with DCG066,the level of reactive oxygen species increased,and the mRNA expression levels of ferroptosis-related genes such as HOMX1,NOX2 and SSAT2 were up-regulated.Conclusion:Histone methyltransferase DCG066 exhibits cytotoxicity to diffuse large B-cell lymphoma by inducing cell ferroptosis and apoptosis,and inhibiting the cell cycle.

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Memo

Memo:
山东省医药卫生科技发展计划（编号：2018WS402）；中国博士后基金项目（编号：2018M632687）
Last Update: 1900-01-01