|Table of Contents|

Knockdown of USP39 gene inhibits the proliferation of human bladder cancer cells and promotes cell apoptosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 05
Page:
791-796
Research Field:
Publishing date:

Info

Title:
Knockdown of USP39 gene inhibits the proliferation of human bladder cancer cells and promotes cell apoptosis
Author(s):
LI Jing; QU Weixing; CHENG Yongyi
Department of Urology,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China.
Keywords:
ubiquitin specific peptidase 39; bladder cancer; proliferation; apoptosis; RNA interference
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2022.05.007
Abstract:
Objective:To investigate the effect of lentivirus-mediated RNA interference knockdown ubiquitin specific peptidase 39(USP39) on the growth and apoptosis of human bladder cancer cells,and to provide new ideas for bladder cancer treatment.Methods:RT-qPCR determined the expression of USP39 mRNA in 5637 and T24 cell lines.Specific shRNA for USP39 was designed and synthesized.Bladder cancer cells were transfected with liposomes.RT-qPCR and Western blotting was used to detect the shRNA inhibition efficiency.Celigo cell counting assay,MTT method and colony-forming ability were used to detect the proliferation and colony-forming ability of cells.Flow cytometry was used to detect apoptosis.Western blotting was used to detect the expression of apoptosis markers C-PARP1,p53,p-p53 and C-Caspase-3.Results:It was determined by RT-qPCR and Western blotting that the expression levels of USP39 mRNA and protein in USP39 knockdown cells were significantly reduced.Through MTT analysis,colony formation analysis and flow cytometry analysis,it was confirmed that the knockdown of USP39 significantly reduced the proliferation and colony formation ability of bladder cancer cells,and induced apoptosis.In addition,Western blotting analysis showed that USP39 gene knockdown may induce apoptosis by up-regulating the expression of p53,p-p53,C-PARP1 and C-Caspase-3 in bladder cancer 5637 cells.Conclusion:Knockdown of USP39 can inhibit the proliferation of bladder cancer cells by activating the Caspase cascade and up-regulating the expression of p53.These data can provide experimental basis for the development of USP39 as a potential molecular target against bladder cancer.USP39 may be a new biomarker for targeted therapy of bladder cancer,and further research is needed.

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Last Update: 2022-01-27