|Table of Contents|

Up-regulation of miR-145 expression inhibits invasion and migration of ovarian cancer HO8910 cells and promotes TβR-Ⅱ protein expression

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 05
Page:
779-784
Research Field:
Publishing date:

Info

Title:
Up-regulation of miR-145 expression inhibits invasion and migration of ovarian cancer HO8910 cells and promotes TβR-Ⅱ protein expression
Author(s):
QI Zhenjun1; DAI Zhiqin2
1.Department of Laboratory;2.Department of Gynecology and Oncology,Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University,Jiangsu Nanjing 210009,China.
Keywords:
OC; miR-145; TβR-Ⅱ; HO8910 cells
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2022.05.005
Abstract:
Objective:To understand the effect of miR-145 on the invasion and migration of OC HO8910 cells and the changes of TβR-Ⅱ protein.Methods:OC HO9810 cells provided by one laboratory cell bank were divided into ZW group(HO8910 cells),ZN group(miR-145-NC),and YJ group(miR-145 mimics).qRT-PCR,MTT,Hoechst33258 fluorescence staining,Transwell cell,cell scratches,Western blot detected miR-145,TβR-Ⅱ expression,cell viability,apoptosis,invasion,migration ability and TβR-Ⅱ protein.Results:The expression level of miR-145 in HO8910 cells in the YJ group was the highest,indicating successful transfection.The expression of TβR-Ⅱ in the YJ group was different from that of the other two groups(P＜0.05).The mRNA expression levels of miR-145 and TβR-Ⅱ in the ZW group and ZN group were the same(P＞0.05).After 48,72,and 96 hours of culture,the activity of HO8910 cells in the YJ group was the weakest compared to the ZW group and the ZN group(P＜0.05).With the continuation of time,the cell viability of the three groups after 96 h culture was better than 48 and 72 h(P＜0.05).The apoptosis rate of HO8910 cells in the YJ group was higher than that in the ZW group and ZN group(P＜0.05).The HO8910 cells in the ZW group had the weakest fluorescence intensity and the lowest apoptosis rate.The ZW group was the same as the ZN group(P＞0.05).The number of cell invasion and migration in the three groups was quite different(P＜0.05).The number of invasions and migrations in the YJ group was less than the other two groups(all P＜0.05).There was no significant difference in the number of cell invasion and migration between ZN group and ZW group(P＞0.05).The expression of TβR-Ⅱ protein in the YJ group was significantly higher than that in the ZW group and ZN group(P＜0.05).The expression of TGF-β protein in the YJ group was reduced compared with the other two groups(P＜0.05),and the expression levels of TGF-β and TβR-Ⅱ proteins in ZW group were less different from those of ZN group(P＞0.05).Conclusion:Up-regulating miR-145 can play a positive role in inhibiting the biological activities of OC cells migration and invasion,which may be related to the targeted regulation of TβR-Ⅱ protein.

References:

[1]张爽爽,夏庆民,郑荣寿,等.中国2010年卵巢癌发病与死亡分析［J］.中国肿瘤,2016,25(3):169-173. ZHANG SS,XIA QM,ZHENG RS,et al.Analysis of the incidence and mortality of ovarian cancer in China in 2010［J］.Chinese Cancer,2016,25(3):169-173.
[2]YEN NGELA,JIMNEZ MARTNEZ YAIZA,JUAN M，et al.Recent progress in gene therapy for ovarian cancer［J］.International Journal of Molecular Sciences,2018,19(7):1930-1937.
[3]TWOROGER SS,DOHERTY JA.Epidemiologic paradigms for progress in ovarian cancer research［J］.Cancer Causes & Control,2017,28(5):361-364.
[4]陈藕景,祝莹.血清肿瘤标志物在卵巢癌早期诊断中的临床价值［J］.中国性科学,2016,25(03):48-50. CHEN OJ,ZHU Y.The clinical value of serum tumor markers in the early diagnosis of ovarian cancer［J］.Chinese Journal of Sexuality,2016,25(03):48-50.
[5]刘康生,陈娟,孙二虎,等.血清人附睾上皮分泌蛋白4、CA125和卵巢恶性肿瘤风险模型在卵巢癌疾病中的诊断价值［J］.中国临床保健杂志,2016,19(01):58-60,541. LIU KS,CHEN J,SUN EH,et al.The diagnostic value of serum human epididymal secretory protein 4,CA125 and ovarian malignant tumor risk model in ovarian cancer［J］.Chinese Journal of Clinical Healthcare,2016,19(01):58-60,541.
[6]吴梦,陈献,娄鉴芳,等.TGF-β1调控卵巢癌微环境中CD8+Treg的表型及功能初探［J］.免疫学杂志,2016，32(12):1058-1062. WU M,CHEN X,LOU JF,et al.The phenotype and function of CD8+Treg in the microenvironment of ovarian cancer regulated by TGF-β1［J］.Chinese Journal of Immunology,2016，32(12):1058-1062.
[7]余俊,李晓兰,张青冬.上皮性卵巢癌组织miR-320a表达对SKOV3细胞增殖和侵袭性影响［J］.中华肿瘤防治杂志,2019,26(14):1026-1030,545. YU J,LI XL,ZHANG QD.The effect of miR-320a expression in epithelial ovarian cancer tissue on the proliferation and invasion of SKOV3 cells［J］.Chinese Journal of Cancer Prevention and Treatment,2019,26(14):1026-1030,545.
[8]冯云云,武春艳,詹瑛.miR-145在浆液性卵巢癌中的表达及对卵巢癌SKOV3细胞增殖的影响［J］.现代肿瘤医学,2018,26(24):42-46. FENG YY,WU CY,ZHAN Y.The expression of miR-145 in serous ovarian cancer and its effect on the proliferation of ovarian cancer SKOV3 cells［J］.Modern Oncology,2018,26(24):42-46.
[9]王禹涵,李佩玲.卵巢癌肿瘤标志物的研究进展［J］.现代肿瘤医学,2018,26(12):163-166. WANG YH,LI PL.Research progress of tumor markers in ovarian cancer［J］.Modern Oncology,2018,26(12):163-166.
[10]洪澜,李成学,贺国丽,等.卵巢癌生物治疗的现状与进展［J］.现代生物医学进展,2016，16(6):1180-1183. HONG L,LI CX,HE GL,et al.Current status and progress of biotherapy for ovarian cancer［J］.Advances in Modern Biomedicine,2016，16(6):1180-1183.
[11]高梅.MRI检查在卵巢肿瘤诊断及鉴别诊断中的临床价值研究［J］.中国CT和MRI杂志,2018,16(01):118-120. GAO M.The clinical value of MRI in the diagnosis and differential diagnosis of ovarian tumors［J］.Chinese Journal of CT and MRI,2018,16(01):118-120.
[12]边策,綦小蓉,姚奎,等.新辅助化疗对晚期卵巢癌治疗应用价值的评估及多因素分析［J］.实用妇产科杂志,2016,32(4):266-271. BIAN C,QI XR,YAO K,et al.Evaluation and multivariate analysis of the application value of neoadjuvant chemotherapy for advanced ovarian cancer［J］.Journal of Practical Obstetrics and Gynecology,2016,32(4):266-271.
[13]胡军梅,郑威.肿瘤标志物检测在卵巢恶性肿瘤筛查中的临床意义［J］.中国肿瘤临床与康复,2017，24(12):78-80. HU JM,ZHENG W.The clinical significance of tumor marker detection in the screening of ovarian malignant tumors［J］.Chinese Journal of Clinical Oncology and Rehabilitation,2017,24(12):78-80.
[14]林晓婷,于楠,高倩倩,等.卵巢癌分子靶向治疗研究进展［J］.中国医师进修杂志,2019,42(9):850-854. LIN XT,YU N,GAO QQ,et al.Research progress in molecular targeted therapy of ovarian cancer［J］.Chinese Journal of Postgraduates of Medicine,2019,42(9):850-854.
[15]叶旭.miRNA在卵巢癌早期诊断和预后中作用的研究进展［J］.中国肿瘤生物治疗杂志,2019,26(6):715-719. YE X.Research progress on the role of miRNA in the early diagnosis and prognosis of ovarian cancer［J］.Chinese Journal of Cancer Biotherapy,2019,26(6):715-719.
[16]CUI X，DI W.Progress in nanoscale RNA delivery systems for the treatment of ovarian cancer［J］.Tumor,2018,38(2):152-156.
[17]ZHANG R，JIAO J，CHU H，et al.Expression of microRNA-145,OCT4,and SOX2 in double primary endometrioid endometrial and ovarian carcinomas［J］.Histology and Histopathology:Cellular and Molecular Biology,2018,33(8):859-870.
[18]袁亚男，杜梦楠，黄艳洁，等.结肠癌细胞中miR-145-5p、miR-143-3p的表达变化对多药耐药的影响［J］.现代肿瘤医学,2019, 27(19):3592-3596. YUAN YN,DU MN,HUANG YJ,et al.Effects of expression changes of miR-145-5p and miR-143-3p in colon cancer cells on multidrug resistance［J］.Modern Oncology,2019, 27(19):3592-3596.
[19]王丹,王莉,王治洁.卵巢癌患者血清肿瘤干细胞标志物CD133和miR-145的临床价值［J］.中国妇幼健康研究,2016,27(4):460-462. WANG D,WANG L,WANG ZJ.The clinical value of serum tumor stem cell markers CD133 and miR-145 in patients with ovarian cancer［J］.Chinese Journal of Woman and Child Health Research,2016,27(4):460-462.
[20]李洁,周园园,赵乐,等.微小RNA-145对卵巢癌细胞迁移和侵袭的影响［J］.中国医学科学院学报,2019,2(5):581-588. LI J,ZHOU YY,ZHAO L,et al.Effects of microRNA-145 on the migration and invasion of ovarian cancer cells［J］.Journal of Chinese Academy of Medical Sciences,2019,2(5):581-588.
[21]夏冰,卢建.地塞米松促进卵巢癌细胞系p21/WAF1和TβR-II的表达［J］.基础医学与临床,2003,23(04):416-419. XIA B,LU J.Dexamethasone promotes ovarian cancer cell lines p21/WAF1 and TβR-II expression［J］.Basic Medicine and Clinic,2003，23(04):416-419.
[22]高洁凡,王东晖,冯静,等.FAP与TGF-β1在卵巢癌中的表达及临床意义［J］.中国妇幼健康研究,2016,27(S2):209-210. GAO JF,WANG DH,FENG J,et al.FAP and TGF- β expression and clinical significance of 1 in ovarian cancer［J］.China Maternal and Child Health Research,2016,27(S2):209-210.

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Last Update: 2022-01-27