|Table of Contents|

miR-21 targeted suppressor TIMP3 expression promotes the invasion of hepatocellular carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 05
Page:
768-772
Research Field:
Publishing date:

Info

Title:
miR-21 targeted suppressor TIMP3 expression promotes the invasion of hepatocellular carcinoma cells
Author(s):
SHI Hui; CAI Duxiong
Department of Gastroenterology,the First Affiliated Hospital of Hainan Medical University,Hainan Haikou 570102,China.
Keywords:
hepatocellular carcinoma; invasion; miR-21; tissue metalloproteinase inhibitor factor 3
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2022.05.003
Abstract:
Objective:To explore miR-21 expression in hepatocellular carcinoma(HCC) cells and its mechanism of regulating cell proliferation and invasion.Methods:The expression of miR-21 was detected by Real-time PCR in HCC(Hep3B,HuH7,SNU398,Hep3G2) and human normal hepatocyte(THLE2,THLE3).Hep3B and HuH7 cells were transfected with miR-21-inhibitor and miR-NC,respectively,then the proliferation of Hep3B and HuH7 cells were detected by CCK-8 assay and the ability of invasion were detected by Transwell assay.Dual luciferase assay was used to detect miR-21 binding to TIMP3 3'-UTR to regulate the expression of TIMP3,and the expression of TIMP3,matrix metalloproteinase 2 and matrix metalloproteinase 9 were determined by Western blot.Results:miR-21 expression in Hep3B,HuH7,SNU398 and Hep3G2 cells was significantly higher than that in THLE2 and THLE3 cells(P＜0.01).After transfected with miR-21-inhibitor,the invasion of Hep3B and HuH7 cells were effectively inhibited(P＜0.01).However，there's no effect on proliferation.The results of double luciferase assay showed that the activity of luciferase was significantly lower in miR-21-mimic transfected +wild type plasmid group than miR-NC+wild type plasmid group(P＜0.01).Compared with miR-NC+mutant plasmid group,the activity of luciferase was no significant changes in miR-21-mimic transfected+mutant plasmid group.After transfected with miR-21-inhibitor,the expression of TIMP3 was increased and the expression of MMP2 and MMP9 was decreased in Hep3B and HuH7 cells,which were significantly different from miR-NC(P＜0.01).Conclusion:miR-21 targeted inhibition the expression TIMP3 to promote the invasion of HCC cells by up-regulating the expression and activation of MMP2 and MMP9.

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Memo

Memo:
海南省自然科学基金资助项目(编号：818MS145)
Last Update: 2022-01-27