|Table of Contents|

Expression and clinical significance of FASN in bladder cancer based on bioinformatics analysis and immunohistochemistry

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 02
Page:
261-267
Research Field:
Publishing date:

Info

Title:
Expression and clinical significance of FASN in bladder cancer based on bioinformatics analysis and immunohistochemistry
Author(s):
YUAN Chao; WANG Lei; DU Yang; CHEN Zhiyuan; LIU Xiuheng
Department of Urology，Renmin Hospital of Wuhan University，Hubei Wuhan 430060，China.
Keywords:
fatty acid synthase; bladder cancer; bioinformatics; immunohistochemistry; clinical significance
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2022.02.017
Abstract:
Objective：To analyze the expression and clinical significance of fatty acid synthase(FASN) in bladder cancer by bioinformatics methods and immunohistochemistry．Methods:The differential expressions of FASN mRNA in bladder cancer tissues and normal bladder tissues were analyzed by using Oncomine database,and Meta-analysis was performed.The paraffin-embedded specimens of 86 patients with bladder cancer undergoing surgery from January 2019 to March 2021 were collected.The expressions of FASN protein in badder cancer and para-cancerous tissues were detected by immunohistochemical staining,and the relationship between FASN protein expression and several clinicopathological characteristics was analyzed.The relationship between FASN mRNA expression and clinicopathological characteristics was analyzed by using LinkedOmics database.The relationship between FASN and the survival of bladder cancer patients was analyzed based on UALCAN and LinkedOmics databases.Enrichment analysis was performed based on LinkedOmics and Metascape databases.Constructed a protein-protein interaction network by using String database.Results:Compared with normal bladder tissues,the expression level of FASN mRNA was higher in bladder cancer tissues.Immunohistochemical examination showed that FASN protein was highly expressed in bladder cancer tissues.The expression of FASN mRNA in bladdder cancer patients was correlated with tumor purity and years to birth (P＜0.05),but not with pathological grade and TNM stage (P＞0.05).The expression of FASN protein in bladdder cancer patients was correlated with age and gender (P＜0.05),but not with pathological grade,myometrial infiltration and lymph node metastasis(P＞0.05).The overall survival of bladder cancer patients with high expression of FASN was significantly lower than that of patients with low expression (P＜0.05).FASN-positively correlated genes were mainly associated with signaling pathways such as activation of gene expression by SREBF,coenzyme A metabolic process,cellular lipid catabolic process and hormone secretion.Conclusion:FASN is over-expressed in bladder cancer tissues,and is associated with poor prognosis.FASN could a new biomarker for the diagnosis and treatment of bladder cancer.

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Memo

Memo:
National Natural Science Foundation of China(No.81972408）；国家自然科学基金面上项目（编号：81972408）；湖北省武汉市应用基础前沿项目（编号：2018060401011321）
Last Update: 1900-01-01