|Table of Contents|

Phenotypic changes and functional analysis of tumor infiltrating dendritic cells in esophageal cancer tissues

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 02
Page:
251-255
Research Field:
Publishing date:

Info

Title:
Phenotypic changes and functional analysis of tumor infiltrating dendritic cells in esophageal cancer tissues
Author(s):
WANG Kun; LYU Shengxiang
Department of Gastroenterology,Lianyungang First People's Hospital,Jiangsu Lianyungang 222061,China.
Keywords:
esophageal cancer; tumor infiltrating dendritic cells; T cell subsets; phenotype
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2022.02.015
Abstract:
Objective：To investigate the phenotype and function of tumor infiltrating dendritic cells in esophageal cancer.Methods:From January 2017 to September 2018,92 patients with esophageal cancer admitted to our hospital were prospectively collected as the observation group,and 92 patients with benign esophageal masses admitted to our hospital during the same period were selected as the control group.Intraoperative samples of esophageal cancer,adjacent tissues and benign masses were taken.The expression level and phenotype of tumor infiltrating dendritic cells in the above tissues were detected.The expression of T cell subsets was detected as well.The correlations between tumor infiltrating dendritic cells and clinical characteristics of esophageal cancer patients were studied.Results:When compared with the control group,the density of tumor infiltrating dendritic cells,the percentage of MHC-II positive dendritic cells and CD54 positive dendritic cells in the observation group were significantly decreased (P＜0.05).The CD4+T cells in the observation group were increased ［(24.81±3.72）% vs （20.77±3.63）%,P=0.000］.CD8+T cells in the observation group were decreased ［(20.90±4.12）% vs （23.08±4.42）%,P=0.001］.The density of tumor infiltrating dendritic cells,the percentage of MHC-II positive dendritic cells and CD54 positive dendritic cells in esophageal cancer were correlated with tumor diameter,TNM stage and lymph node metastasis (P＜0.05).The density of tumor infiltrating dendritic cells,MHC-II positive dendritic cells and CD54 positive dendritic cells were negatively correlated with CD4+T cells and positively correlated with CD8+T cells in esophageal cancer tissues (P＜0.05).Conclusion:Tumor infiltrating dendritic fineness is low expression and incompetent in esophageal cancer,which is related to imbalance of T cell subsets and poor prognosis.

References:

［1］刘文,王琳.比较新辅助放化疗联合手术与单纯手术治疗局部晚期食管癌的疗效［J］.中国医药指南,2019,17(23):37. LIU Wen,WANG Lin.To compare the effect of neoadjuvant radiotherapy and chemotherapy combined with operation and operation alone in the treatment of locally advanced esophageal cancer ［J］.Guide of China Medicine,2019,17(23):37.
［2］王冬苗,刘洋.三维适形放射疗法与顺铂同步化疗对晚期食管癌患者副反应的对比观察［J］.湖南师范大学学报(医学版),2018,15(05):178-180. WANG Dongmiao,LIU Yang.Comparison of three-dimensional conformal radiotherapy combined with cisplatin in the treatment of patients with advanced esophageal cancer ［J］.Journal of Hunan Normal University(Medical Science),2018,15(05):178-180.
［3］陈丽丽,周咏春,江浩.老年局部晚期食管癌的研究进展［J］.重庆医科大学学报,2018,43(05):736-740. CHEN Lili,ZHOU Yongchun,JIANG Hao.Research progress of locally advanced esophageal cancer in the elderly［J］.Journal of Chongqing Medical University,2018,43(05):736-740.
［4］ZHANG W,PAN Y,GOU P,et al.Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model［J］.Oncol Rep,2018,39(1):280-288.
［5］LI L,LIU X,SANDERS KL,et al.TLR8-mediated metabolic control of human Treg function:A mechanistic target for cancer immunotherapy［J］.Cell Metab,2018,12(3):781-785.
［6］MENG X,ZHU S,DONG Q,et al.Expression of Th17/Treg related molecules in gastric cancer tissues［J］.Turk J Gastroenterol,2018,29(1):45-51.
［7］MUNN DH,SHARMA MD,JOHNSON TS.Treg destabilization and reprogramming:Implications for cancer immunotherapy［J］.Cancer Res,2018,78(18):5191-5199.
［8］GARG B,GIRI B,MODI S,et al.NFkappaB in pancreatic stellate cells reduces infiltration of tumors by cytotoxic T cells and killing of cancer cells,via up-regulation of CXCL12［J］.Gastroenterology,2018,155(3):880-891.
［9］KERDIDANI D,MAGKOUTA S,CHOUVARDAS P,et al.Cigarette smoke-induced emphysema exhausts early cytotoxic CD8(+) T cell responses against nascent lung cancer cells［J］.J Immunol,2018,201(5):1558-1569.
［10］KIMURA H,MATSUI Y,ISHIKAWA A,et al.Randomized controlled phase III trial of adjuvant chemoimmunotherapy with activated cytotoxic T cells and dendritic cells from regional lymph nodes of patients with lung cancer［J］.Cancer Immunol Immunother,2018,67(8):1231-1238.
［11］HONG Y,YAO Y,ZHAO H,et al.Dendritic mesoporous silica nanoparticles with abundant Ti(4+) for phosphopeptide enrichment from cancer cells with 96% specificity［J］.Anal Chem,2018,90(12):7617-7625.
［12］LE MOIGNIC A,MALARD V,BENVEGNU T,et al.Preclinical evaluation of mRNA trimannosylated lipopolyplexes as therapeutic cancer vaccines targeting dendritic cells［J］.J Control Release,2018,278(12):110-121.
［13］LIU H,CHEN L,PENG Y,et al.Dendritic cells loaded with tumor derived exosomes for cancer immunotherapy［J］.Oncotarget,2018,9(2):2887-2894.
［14］LIU W,ZHAO J,LI Q,et al.Gastric cancer patients have elevated plasmacytoid and CD1c(+) dendritic cells in the peripheral blood［J］.Oncol Lett,2018,15(4):5087-5092.
［15］MICHEA P,NOEL F,ZAKINE E,et al.Adjustment of dendritic cells to the breast-cancer microenvironment is subset specific［J］.Nat Immunol,2018,19(8):885-897.
［16］OGASAWARA M,MIYASHITA M,OTA S.Vaccination of urological cancer patients with WT1 peptide-pulsed dendritic cells in combination with molecular targeted therapy or conventional chemotherapy induces immunological and clinical responses［J］.Ther Apher Dial,2018,22(3):266-277.
［17］MOOKERJEE A,GRACIOTTI M,KANDALAFT L.A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNalpha and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model［J］.Bioimpacts,2018,8(3):211-221.
［18］TAKAKURA K,KOIDO S,KAN S,et al.Prognostic markers for patient outcome following vaccination with multiple MHC Class I/II-restricted WT1 peptide-pulsed dendritic cells plus chemotherapy for pancreatic cancer［J］.Anticancer Res,2015,35(1):555-562.
［19］MAZORRA Z,LAVASTIDA A,CONCHA-BENAVENTE F,et al.Nimotuzumab induces NK cell activation,cytotoxicity,dendritic cell maturation and expansion of EGFR-specific T cells in head and neck cancer patients［J］.Front Pharmacol,2017,8(2):382-386.
［20］MARIGO I,ZILIO S,DESANTIS G,et al.T cell cancer therapy requires CD40-CD40L activation of tumor necrosis factor and inducible nitric-oxide-synthase-producing dendritic cells［J］.Cancer Cell,2016,30(3):377-390.
［21］CORREALE P,MICHELI L,VECCHIO MT,et al.A parathyroid-hormone-related-protein (PTH-rP)-specific cytotoxic T cell response induced by in vitro stimulation of tumour-infiltrating lymphocytes derived from prostate cancer metastases,with epitope peptide-loaded autologous dendritic cells and low-dose IL-2［J］.Br J Cancer,2001,85(11):1722-1730.
［22］MARTINET L,FILLERON T,LE GUELLEC S,et al.High endothelial venule blood vessels for tumor-infiltrating lymphocytes are associated with lymphotoxin beta-producing dendritic cells in human breast cancer［J］.J Immunol,2013,191(4):2001-2008.
［23］SMITH LE,OLSZEWSKI MA,GEORGOUDAKI AM,et al.Sensitivity of dendritic cells to NK-mediated lysis depends on the inflammatory environment and is modulated by CD54/CD226-driven interactions［J］.J Leukoc Biol,2016,100(4):781-789.
［24］YAN J,LIU B,SHI Y,et al.Class II MHC-independent suppressive adhesion of dendritic cells by regulatory T cells in vivo［J］.J Exp Med,2017,214(2):319-326.
［25］SPALLANZANI RG,TORRES NI,AVILA DE,et al.Regulatory dendritic cells restrain NK cell IFN-gamma production through mechanisms involving NKp46,IL-10,and MHC Class I-specific inhibitory receptors［J］.J Immunol,2015,195(5):2141-2148.
［26］MA XJ,PAN XL,LYV ZH,et al.Therapeutic influence on circulating and monocyte-derived dendritic cells in laryngeal squamous cell carcinoma patients［J］.Acta Otolaryngol,2009,129(1):84-91.
［27］LIANG W,WANG XF.In vitro induction of specific anti-tumoral immunity against laryngeal carcinoma by using human interleukin-12 gene-transfected dendritic cells［J］.Chin Med J (Engl),2011,124(9):1357-1361.
［28］KIYOMI A,MAKITA M,OZEKI T,et al.Characterization and clinical implication of Th1/Th2/Th17 cytokines produced from three-dimensionally cultured tumor tissues resected from breast cancer patients［J］.Transl Oncol,2015,8(4):318-326.
［29］MA J,LIU H,WANG X.Effect of ginseng polysaccharides and dendritic cells on the balance of Th1/Th2 T helper cells in patients with non-small cell lung cancer［J］.J Tradit Chin Med,2014,34(6):641-645.
［30］HONG M,JIANG Z,ZHOU YF.Effects of thermotherapy on Th1/Th2 cells in esophageal cancer patients treated with radiotherapy［J］.Asian Pac J Cancer Prev,2014,15(5):2359-2362.

Memo

Memo:
-
Last Update: 1900-01-01