|Table of Contents|

NuSAP1 promotes the proliferation and invasion of triple-negative breast cancer cells through the Wnt/β-catenin/EMT pathway and its predictive value for prognosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 02
Page:
235-242
Research Field:
Publishing date:

Info

Title:
NuSAP1 promotes the proliferation and invasion of triple-negative breast cancer cells through the Wnt/β-catenin/EMT pathway and its predictive value for prognosis
Author(s):
SUN Li; ZHAO Yi
Department of Breast Surgery,Shengjing Hospital of China Medical University,Liaoning Shenyang 110022,China.
Keywords:
NuSAP1; triple-negative breast cancer; Wnt/β-catenin pathway; epithelial-mesenchymal transition pathway; cell proliferation; cell invasion
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2022.02.012
Abstract:
Objective：We analyzed the effect of expression of nucleolar spindle-associated protein 1 (NuSAP1) on the prognosis of breast cancer (BC) and investigated the potential mechanism of tumorigenicity of triple-negative breast cancer (TNBC) cell line.Methods:We downloaded the RNA-seq breast cancer (BC) data from The Cancer Genome Atlas (TCGA),and screened the NuSAP1 gene using R software.The clinical data for patients with BC were screened and analyzed with R software.The survival curve was drawn using the Kaplan-Meier-plotter.Cell proliferation and invasion were verified by cell counting kit-8 and Transwell assays.Expression of NuSAP1,Wnt/β-catenin pathway and epithelial-mesenchymal-transition-related proteins in TNBC was detected by real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting (WB).Results:The expression of NuSAP1 was upregulated in BC.The difference in NuSAP1 expression was related to multiple clinicopathological factors,and the higher expression of NuSAP1,the shorter the survival time.In MDA-MB-231 and BT-549 cells,when NuSAP1 was knocked down,cell proliferative and invasive ability decreased.Expression of CyclinD1,Vimentin,Slug,Twist,Wnt3a and p-β-catenin decreased.And expression of E-cadherin increased significantly.The results of the sh-NuSAP1+ov-NuSAP1 group were opposite from the results of the sh-NuSAP1 group.Conclusion:NuSAP1 is a carcinogen that facilitates the progress of TNBC,through the Wnt/β-catenin and epithelial-mesenchymal transition pathway.

References:

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