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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2022 02

Page:

230-234

Research Field:

Publishing date:

Info

Title:

Expression of hsa_circ_0064369 in esophageal squamous cell carcinoma tissues and cells and its clinical significance

Author(s):

CAO Shasha¹; LI Junkuo²; DUAN Lijuan¹; XIA Jin³; YANG Haijun²; ZHOU Fuyou⁴

1.Department of Central Laboratory;2.Department of Pathology;3.The Fifth Department of Medical Oncology;4.Department of Thoracic Surgery,Anyang Tumor Hospital,the Fourth Affiliated Hospital of Henan University of Science and Technology,Henan Anyang 455000

Keywords:

circRNA; esophageal squamous cell carcinoma; RT-qPCR; hsa_circ_0064369; molecular biomarker

PACS:

R735.1

DOI:

10.3969/j.issn.1672-4992.2022.02.011

Abstract:

Objective：To analyze the expression of hsa_circ_0064369 in esophageal squamous cell carcinoma and its clinical significance.Methods:The expression of circRNA was detected with circRNA microarray assay in six samples of ESCC tumor and matched adjacent tissues.Based on the following four criterion,including:Fold Change≥5,P＜0.01,high consistence in samples,high original signal value,hsa_circ_0064369 was choosed as the object of study.Real-time quantitative polymerase chain reaction was uesd to detect the expression of hsa_circ_0064369 in 43 pair ESCC tissues and normal adjacent tissues,also in normal esophageal epithelial cell (NE6) and esophageal carcinoma cell lines (NE3,KYSE9706).The relationship between the expression of hsa_circ_0064369 and its clinicopathological characteristics was analysed.A receiver operating characteristic (ROC) curve was established to evaluate the sensitivity and specificity of hsa_circ_0064369 in the diagnosis of ESCC.Results:The expression of hsa_circ_0064369 in the ESCC tissues(0.35±0.41) was lower than that in corresponding adjacent normal tissues（1.88±1.22）（P＜0.01）.The expression level of hsa_circ_0064369 in two kinds of esophageal carcinoma cell lines（0.46±0.18，0.53±0.09） were lower than that in normal esophageal cell line（1.00±0.37）（P＜0.05）.hsa_circ_0064369 expression level was associated with TNM stage（P=0.022）,but showed no significant differences in gender,age,differentiated degree,tumor diameter,tumor position and lymph node metastasis.The disease free survival time was significantly shorter in patients with lowly expressed hsa_circ_0064369 than that in patients with highly expressed hsa_circ_0064369.The area under the ROC curve was 0.928 and its sensitivity and specificity were 88.4% and 90.7% when the truncation value was 0.699 5.Conclusion:The expression of hsa_circ_0064369 is downregulated in ESCC tissues,indicating a poor prognosis.hsa_circ_0064369 is a potential biomarker for esophageal cancer.

References:

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Memo

Memo:

河南省医学科技攻关计划项目（编号：2018021015）

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